Development of scaffold-free spheroids in agarose micromolds from murine muscle derived stem cells

• Grant number: 20/03609-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2020
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Roberta Sessa Stilhano Yamaguchi
• Grantee: Sofia Mila Cantu de Sales
• Host Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP). Fundação Arnaldo Vieira de Carvalho. São Paulo , SP, Brazil

Abstract

In muscle injuries with big volume loss the flap-free is used as a therapeutic alternative. However, this procedure has several obstacles including the inevitable commitment of the donor source. Spheroids have emerged as a promising alternative for the production of 3D cultures, as they mimic the environment in vivo and can be used to create grafts capable of regenerated tissues and injured organs. An agarose micromold technique, free of scaffolding, which allows cells to produce extracellular matrix, allowing greater cell-cell interaction, recreating the microenvironment and recapitulating tissue morphogenesis. In addition, cell culture for long periods allows greater production yield and greater homogeneity in spheroid size and shape. The production of steroids from muscle derived stem cells (MDSCs) opens therapeutic perspectives in the area of regenerative medicine for muscle injuries. Like MDSCs, they stand out for including the possible use in the treatment of muscle diseases and their potential to promote the regeneration and repair of other tissues. In this way, the objective of the present project is produce spheroids from MDSCs using the scaffold-free technique.