Biochemical characterization of angiotensin (Ang) I without plasma and aorta of C57Bl / 6 and ELA-2KO mice with abdominal aortic aneurysm (AAA) induced by the infusion of II

• Grant number: 20/09999-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2020
• End date: October 31, 2021
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Christiane Becari
• Grantee: Lígia Cristina Borges Campos
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 17/21539-6 - Role of renin angiotensin system in Abdominal Aortic Aneurysm, AP.JP

Abstract

The renin-angiotensin system (RAS) is involved in physiological and pathological processes. Currently, a new paradigm has emerged. It has that two main axes that modulate the SRA function: an axis ACE / Ang II / AT1 receptor, and another ACE2 / Ang- (1-7) / MAS receptor. These functional aspects increase the range of possible targets for the treatment of cardiovascular diseases, including abdominal aortic aneurysm (AAA). Experimental research in mice and rats indicates an essential contribution of RAS to the pathogenesis of AAA. An experimental model of AAA formation is by chronic infusion of Ang II in mice. This model of aneurysm mimics the disease seen in humans with several similar characteristics. Thus, we intend to describe the profile of the Ang I forming activity of Ang II, Ang 1-7, and Ang IV from Ang I in the presence and absence of specific RAS inhibitors, in the plasma and the aorta of these animals by HPLC. Another aspect of the project is related to elastase-2 (ELA-2), and Ang II-forming enzymes in the vascular system. It will be interesting to clarify the contribution of ELA-2 in the development of AAA in mice. Together, the present subprojects intend to evaluate the participation of the RAS components in the pathophysiology of abdominal aortic aneurysm in wild mice (C57 / Bl-6) and elastase-2 knockout (ELA-2 KO) induced by the continuous infusion of Ang II. The experiments proposed in these projects present the experimental groups: I) Mouse C57Bl / 6 Control; II) Mouse Knockout Ela-2 Control; III) C57Bl / 6 mice with AAA; IV) Knockout Ela-2 mice with AAA. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

D'AVILA-MESQUITA, CAROLINA; COUTO, ARIEL E. S.; CAMPOS, LIGIA C. B.; VASCONCELOS, TAUANA F.; MICHELON-BARBOSA, JESSYCA; CORSI, CARLOS A. C.; MESTRINER, FABIOLA; PETROSKI-MORAES, BRUNO C.; GARBELLINI-DIAB, MARIA J.; COUTO, DANIEL M. S.; et al. MMP-2 and MMP-9 levels in plasma are altered and associated with mortality in COVID-19 patients. BIOMEDICINE & PHARMACOTHERAPY, v. 142, OCT 2021. (18/23718-8, 19/24369-0, 21/01195-6, 19/21721-4, 20/09999-4, 19/11485-1, 20/03308-0)