Development of a cellular assay to measure SARS-CoV-2 Nsp3 macrodomain activity in vitro

• Grant number: 20/11162-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2020
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Nicolas Carlos Hoch
• Grantee: Lilian Cristina Russo Vieira
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 20/05317-6 - Inhibition of the viral macrodomain as a strategy for Coronavirus treatment, AP.R

Abstract

The SARS-CoV-2 Nsp3 macrodomain is a promising target for COVID-19 treatment. As part of an international effort to identify viral macrodomain inhibitors, this post-doctoral fellowship is aimed specifically at developing a cellular assay to detect the catalytic activity of the ectopically expressed viral macrodomain in human cells and use this assay to refine the list of candidate compounds for further tests. (AU)