Production of conductive filaments containing carbon nanomaterials and metal nanoparticles for the development of low cost enzymeless electrochemical biosensors via additive manufacturing

• Grant number: 20/00325-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2020
• End date: July 24, 2021
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Lucio Angnes
• Grantee: Diego Pessoa Rocha
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

3D printing is the most popular form of Additive Manufacturing (AM). This technology allows the fabrication of three-dimensional objects with the most varied applications in many fields, especially in the chemistry area. Various commercialized electrochemical devices, such as printed electrodes and mainly electrochemical cells, have relatively high costs. Therefore, AM is promising, from the economic and scientific point of view, in the construction of improved electroanalytical devices. To achieve this, the popularization of 3D printers allowed these devices to be printed quickly at very low costs, requiring only one design (designed in specialized software) and polymeric materials (thermoplastics). In addition to AM making it possible to create new analytical apparatus in non-commercially available configurations, it is possible to print modified conductive electrodes with different materials usable for the analysis of different analytes. Thus, the present project aims at the development of modified filaments (composites) with carbon conductive materials (graphene, carbon black, and carbon nanotubes) and metallic nanoparticles (copper, nickel, and gold), from a filament extruder, for the manufacture of low cost enzymeless electrochemical biosensors for the selective determination of analytes of biologic importance, among them glucose, uric acid and lactate in biologic samples. In the manufacture of these new sensors will be evaluated the best proportions between modifying materials and thermoplastics, taking into account the electrochemical response and printability. The characterization of the modified filaments will be performed through techniques such as microscopy, Raman, infrared, electrochemical impedance spectroscopy, among others. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, JOSUE M.; ROCHA, DIEGO P.; SILVA, MURILLO N. T.; MARTINS, PAULO R.; NOSSOL, EDSON; ANGNES, LUCIO; ROUT, CHANDRA SEKHAR; MUNOZ, RODRIGO A. A.. Feasible strategies to promote the sensing performances of spinel MCo2O4 (M = Ni, Fe, Mn, Cu and Zn) based electrochemical sensors: a review. JOURNAL OF MATERIALS CHEMISTRY C, v. 9, n. 25, p. 7852-7887, JUL 7 2021. (17/13137-5, 18/16896-7, 14/50867-3, 20/00325-0)

GONCALVES, JOSUE M.; DE FARIA, LUCAS, V; NASCIMENTO, AMANDA B.; GERMSCHEIDT, RAFAEL L.; PATRA, SANTANU; HERNANDEZ-SARAVIA, LUCAS P.; BONACIN, JULIANO A.; MUNOZ, RODRIGO A. A.; ANGNES, LUCIO. Sensing performances of spinel ferrites MFe2O4 (M = Mg, Ni, Co, Mn, Cu and Zn) based electrochemical sensors: A review. Analytica Chimica Acta, v. 1233, p. 31-pg., 2022-10-22. (13/22127-2, 18/16896-7, 17/13137-5, 20/00325-0, 14/50867-3)

GONCALVES, JOSUE M.; SILVA, MURILLO N. T.; NAIK, KUSHA KUMAR; MARTINS, PAULO R.; ROCHA, DIEGO P.; NOSSOL, EDSON; MUNOZ, RODRIGO A. A.; ANGNES, LUCIO; ROUT, CHANDRA SEKHAR. Multifunctional spinel MnCo₂O₄ based materials for energy storage and conversion: a review on emerging trends, recent developments and future perspectives. JOURNAL OF MATERIALS CHEMISTRY A, v. 9, n. 6, p. 31-pg., 2021-02-16. (18/16896-7, 20/00325-0, 17/13137-5, 14/50867-3)

GONCALVES, JOSUE M.; MARTINS, PAULO R.; ROCHA, DIEGO P.; MATIAS, TIAGO A.; JULIAO, MURILO S. S.; MUNOZ, RODRIGO A. A.; ANGNES, LUCIO. Recent trends and perspectives in electrochemical sensors based on MOF-derived materials. JOURNAL OF MATERIALS CHEMISTRY C, v. 9, n. 28, p. 8718-8745, JUL 28 2021. (14/50867-3, 17/13137-5, 18/16896-7, 20/00325-0)

GONCALVES, JOSUE M.; LIMA, IRLAN S.; AZEREDO, NATHALIA F. B.; ROCHA, DIEGO P.; DE SIERVO, ABNER; ANGNES, LUCIO. iVCe-Layered Double Hydroxide as Multifunctional Nanomaterials for Energy and Sensor Application. FRONTIERS IN MATERIALS, v. 8, NOV 29 2021. (17/13137-5, 20/00325-0, 07/08244-5, 07/54829-5, 18/16896-7, 14/50867-3)

SANTOS, BERLANE G.; GONCALVES, JOSUE M.; ROCHA, DIEGO P.; HIGINO, GIANE S.; YADAV, THAKUR P.; PEDROTTI, JAIRO J.; AJAYAN, PULICKEL M.; ANGNES, LUCIO. Electrochemical sensor for isoniazid detection by using a WS2/CNTs nanocomposite. SENSORS AND ACTUATORS REPORTS, v. 4, NOV 2022. (19/22126-2, 12/50259-8, 18/16896-7, 17/13137-5, 14/50867-3, 20/00325-0)

ATAIDE, VANESSA N.; ROCHA, DIEGO P.; DE SIERVO, ABNER; PAIXAO, THIAGO R. L. C.; MUNOZ, RODRIGO A. A.; ANGNES, LUCIO. Additively manufactured carbon/black-integrated polylactic acid 3Dprintedsensor for simultaneous quantification of uric acid and zinc in sweat. Microchimica Acta, v. 188, n. 11, NOV 2021. (18/14462-0, 20/00325-0, 07/08244-5, 07/54829-5, 14/50867-3)

ROCHA, DIEGO P.; ATAIDE, VANESSA N.; DE SIERVO, ABNER; GONSALVES, JOSUE M.; MUNOZ, RODRIGO A. A.; PAIXA, THIAGO R. L. C.; ANGNES, LUCIO. Reagentless and sub-minute laser-scribing treatment to produce enhanced disposable electrochemical sensors via additive manufacture. CHEMICAL ENGINEERING JOURNAL, v. 425, DEC 1 2021. (07/08244-5, 18/14462-0, 07/54829-5, 18/16896-7, 14/50867-3, 18/08782-1, 20/00325-0)

PEREIRA, JIAN F. S.; ROCHA, RAQUEL G.; CASTRO, SILVIA V. F.; JOAO, AFONSO F.; BORGES, PEDRO H. S.; ROCHA, DIEGO P.; DE SIERVO, ABNER; RICHTER, EDUARDO M.; NOSSOL, EDSON; GELAMO, V, ROGERIO; et al. Reactive oxygen plasma treatment of 3D-printed carbon electrodes towards high-performance electrochemical sensors. SENSORS AND ACTUATORS B-CHEMICAL, v. 347, NOV 15 2021. (20/00325-0, 07/08244-5, 07/54829-5)

STEFANO, JESSICA SANTOS; KALINKE, CRISTIANE; DA ROCHA, RAQUEL GOMES; ROCHA, DIEGO PESSOA; OLIANI PEDRO DA SILVA, VINICIUS APARECIDO; BONACIN, JULIANO ALVES; ANGNES, LUCIO; RICHTER, EDUARDO MATHIAS; JANEGITZ, BRUNO CAMPOS; ABARZA MUNOZ, RODRIGO ALEJANDRO. Electrochemical (Bio)Sensors Enabled by Fused Deposition Modeling-Based 3D Printing: A Guide to Selecting Designs, Printing Parameters, and Post-Treatment Protocols. Analytical Chemistry, v. 94, n. 17, p. 13-pg., 2022-05-03. (17/21097-3, 13/22127-2, 20/00325-0)

GONCALVES, JOSUE M.; SILVA, MURILLO N. T.; NAIK, KUSHA KUMAR; MARTINS, PAULO R.; ROCHA, DIEGO P.; NOSSOL, EDSON; MUNOZ, RODRIGO A. A.; ANGNES, LUCIO; ROUT, CHANDRA SEKHAR. Multifunctional spinel MnCo2O4 based materials for energy storage and conversion: a review on emerging trends, recent developments and future perspectives. JOURNAL OF MATERIALS CHEMISTRY A, v. 9, n. 6, p. 3095-3124, FEB 14 2021. (14/50867-3, 20/00325-0, 18/16896-7, 17/13137-5)