Subtle alterations triggered by nanoparticles analyzed by image-based multiparametric single-cell profiling

• Grant number: 19/24033-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2020
• End date: April 30, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Marcelo Bispo de Jesus
• Grantee: Fernanda Garcia Fóssa
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 22/01483-4 - Subtle alterations triggered by nanoparticles \analyzed by image-based multiparametric single-cell profiling, BE.EP.DR

Abstract

Nanomaterials are present in the everyday life, across products for home, and medical applications. The widespread of nanomaterials in the environment have caused the accumulation in water and biosolids at low concentrations, which can cause human intoxication and alterations from a lifetime exposure. However, nanotoxic studies have focused on cell death caused by high concentrations. Our project aims to investigate subtle alterations triggered by exposure to nanoparticles at low concentrations. To detect these alterations, we will analyse the phenotypic profiling generated from image-based multiparametric single-cell analysis. The analysis and grouping of these parameters will be done through the use of data science tools like machine learning. This approach aims to develop a platform to identify subtle alterations by a variety of nanoparticles, contributing with the risk evaluation and the theoretical basis for the development of therapeutic management strategies.