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This exchange proposal has a primary objective of establishing a collaborative international research consortium specializing in the fields of nanotechnology and bioimage analysis. We will focus on evaluating both the therapeutic and toxic effects of nanoparticles by incorporating advanced bioimage analysis techniques to propel our research endeavors forward. Through a comprehensive exchange program, we aim to foster collaboration between distinguished institutions: the University of Campinas and the KTH Royal Institute of Technology/Science for Life Laboratory in Sweden. This partnership represents a remarkable opportunity to synergize our ongoing projects, augment their outcomes, and lay the groundwork for future innovative and transformative research endeavors. In pursuit of these goals, we propose a two-year planning grant to expand our experimental work and data analysis. This proposal aligns with other ongoing FAPESP projects (2020/01218-3 and 2023/06143-0) and complements existing student fellowships (2023/05204-5, 2022/09892-0, 2022/09246-1, 2022/07854-4, and 2019/24033-1). In addition, it also intersects with existing funding grants from the partner at KTH to apply cutting-edge methods in analyzing microscopy data. Our team of researchers brings together diverse expertise and a strong commitment to collaboration. Consequently, this proposal's anticipated outcomes include establishing a robust international partnership centered on developing novel assays and innovative approaches to enhance the depth and precision of phenotypic profiling from cells treated with nanoparticles. This initiative lays the foundation for future grant proposals, collaborative projects, exchange visits for students and scholars, and the production of high-impact scientific contributions. By fostering this collaborative path, we are confident to promote significant efforts in advancing our understanding of nanotechnology's potential, ultimately driving forward computational tools to improve and overcome challenges in cell profiling techniques. (AU)

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