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Molecular aspects of the nano-cell interactions: therapeutic activity, delivery of oligonucleotides and nanotoxicity

Grant number:23/06143-0
Support Opportunities:Regular Research Grants
Start date: December 01, 2023
End date: November 30, 2025
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Eneida de Paula
Grantee:Eneida de Paula
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
City of the host institution:Campinas
Associated research grant(s):23/14842-5 - PROBING NANO-CELL INTERACTIONS: INSIGHTS FROM IMAGE-BASED PROFILING, AP.R SPRINT

Abstract

Nanomaterials have enormous potential to solve problems in various fields, such as agriculture, livestock, and medicine. The biological role of nanomaterials is closely related to their physicochemical properties, and because these nanomaterials interact with the environment in which they are inserted, the corona effect appears as a protagonist. An important application of nanomaterials is the delivery of drugs and genes that can be used to achieve various biological goals, such as treating tumors, controlling pests, and genetically improving the cellular microenvironment. However, developing or evaluating nanomaterials without considering their toxic effects is impossible. Therefore, the unintentional exposure to pollutants in the nanometer range, such as silver nanoparticles and micro- and nanoplastics, should be emphasized here. Thus, the assessment of the toxic effects of nanomaterials is essential for nanomaterials that are designed for use in their nanometric form or that have the potential to produce these species. Therefore, this grant is divided into three different subprojects: NanoCell@Therapeutics: which aims to investigate aspects of nanoparticle-cell interaction and understand the mechanisms of using nanomaterials to treat tumors; NanoCell@GeneDelivery: aims to develop gene carriers to control biological pests and improve the development of bovine embryos; NanoCell@Nanotox: aims to assess the molecular mechanisms of toxicity induced by different materials such as micro- and nanoplastics and silver nanoparticles. Finally, by understanding the molecular aspects of the interaction between nanomaterials and cells, we aim to contribute to the conscious use of nanomaterials for therapeutic purposes and the delivery of oligonucleotides, always paying attention to their toxic effects, trying to strike a balance between biological efficiency and toxicity. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (4)
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
DE LIMA, LUCAS FELIPE; FERREIRA, ANDRE LOPES; DO NASCIMENTO, GUIDA HELLEN MOTA; CARDOSO, LUANA PEREIRA; DE JESUS, MARCELO BISPO; DE ARAUJO, WILLIAM REIS. Tongue depressor (bio)sensors: A fast decentralized self-testing of salivary biomarkers for personalized medicine. CHEMICAL ENGINEERING JOURNAL, v. 494, p. 13-pg., . (22/09892-0, 23/03483-4, 23/05204-5, 23/12589-0, 23/06143-0, 23/00246-1, 18/08782-1, 22/03250-7)
GARCIA-FOSSA, FERNANDA; MORAES-LACERDA, THAIS; RODRIGUES-DA-SILVA, MARIANA; DIAZ-ROHRER, BARBARA; SINGH, SHANTANU; CARPENTER, ANNE E.; CIMINI, BETH A.; DE JESUS, MARCELO BISPO. Live-cell painting: Image-based profiling in live cells using acridine orange. MOLECULAR BIOLOGY OF THE CELL, v. 36, n. 7, p. 11-pg., . (19/24033-1, 23/06143-0, 22/01483-4, 20/01218-3)
MENNA, BRUNO L.; SIMIC, MILA; TASIC, LJUBICA; CUNHA, MARIELTON DOS PASSOS; DE JESUS, MARCELO B.. Silver ion toxicity in commercial colloidal silver: molecular insights from high-content imaging in Huh-7 cells. Nanotoxicology, v. N/A, p. 17-pg., . (20/01218-3, 23/06143-0)
RIZOLI, CINTIA; DOS SANTOS, NATHALIA MEDINA; MAROSTICA JUNIOR, MARIO ROBERTO; DA CRUZ-HOFLING, MARIA ALICE; MENDONCA, MONIQUE CULTURATO PADILHA; DE JESUS, MARCELO BISPO. The therapeutic potential of reduced graphene oxide in attenuating cuprizone-induced demyelination in mice. Nanotechnology, v. 36, n. 2, p. 12-pg., . (23/06143-0, 20/01218-3)