The impact of bFGF supplementation in in vitro culture of bovine embryos at inner cell mass differentiation

• Grant number: 20/11596-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2021
• End date: December 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Fábio Gouveia Nogueira
• Grantee: Isabella Maran Pereira
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The bovine embryonic development, both in vivo derived and in vitro produced, involves basic aspects of cell differentiation. Throughout embryonic development, the cells that compose the inner cell mass (ICM) differentiate into two new cell lineages: epiblast (origin of germ-layers) and hypoblast (origin of extraembryonic tissues). The fibroblast growth factor (FGF) acts in differentiation processes, cell surviving and division and participates of signaling pathways, being considered a crucial factor for embryonic development. The supplementation with exogenous FGF inhibits Nanog expression, enhances GATA6 expression and, consequently, blocks the epiblast lineage occurrence favoring hypoblast formation. This model has a potential applicability in cattle, as an alternative of Tetraploid Complementation Assay largely used in mice. The aim of this project is to analyze the effects of FGF addition in in vitro culture (IVC) on production and cell differentiation markers of the blastocyst. Besides that, it will be determined which FGF concentration affects ICM differentiation. Thus, embryos will be produced in vitro and submitted to FGF concentrations (0, 10, 100 or 1,000 ng/mL) on day 5 of IVC - there will be used, at least, 25 oocytes per group in which one of the 15 replicates. The blastocyst and total hatching rates as well as the analysis of abundance of target transcripts (by RT-qPCR) and the immunolocation for differentiation markers of cell lines will be evaluated.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ROSSI, HUGO; BORTOLIERO COSTA, CAMILA; RODRIGUES-ROSSI, LUANA TEIXEIRA; BARROS NUNES, GIOVANA; SPINOSA CHELES, DORIS; MARAN PEREIRA, ISABELLA; ROCHA, DANIELE F. O.; FEITOSA, ELOI; COLNAGHI SIMIONATO, ANA VALERIA; ZOCCAL MINGOTI, GISELE; et al. Modulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v. 50, n. 1, p. 10-pg., 2022-12-31. (20/07634-9, 20/11596-5, 12/50533-2, 19/10732-5)