Inhibition of chaperones with violacein in Saccharomyces cerevisiae

• Grant number: 20/15068-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2021
• End date: February 28, 2022
• Field of knowledge: Biological Sciences - Parasitology
• Principal Investigator: Elizabeth Bilsland
• Grantee: Lucas Jung Eberhardt
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Violacein is a natural compound with antibacterial, antiparasitic and antitumor activity. Several published studies have shown that violacein induces apoptosis, but the mechanism for this has remained unclear. Using a genomic chemical profile in yeasts, we have identified direct targets of violacein: chaperones Hsp70. The present work aims to elucidate the cellular effects of the inhibition of Hsp70 chaperones in yeast cells.