Nano-biological consequences of multi-charged surface silica nanoparticles on the formation of the protein corona, colloidal stability, biocompatibility, and cellular uptake

• Grant number: 19/24894-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2021
• End date: November 12, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Lindomar Jose Calumby Albuquerque
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated research grant: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles, AP.TEM
• Associated scholarship(s): 22/04327-3 - The role of in vivo protein corona formation on immune responses, BE.EP.PD

Abstract

Nanoparticles are promising structures for the development of diagnostic and therapeutic agents. On the other hand, despite the great potential, the current stage of nanoparticles application in the Biomedical field is followed by a therapeutic efficacy lower than 1% when inserted in vivo. This low efficiency has been pointed out as the result of an underestimated comprehension of the interaction between nanoparticles and biological media, mainly due to the new biological identity of nanoparticles when adsorb biomolecules from physiological fluids. In this context, the aim of this study is the systematic evaluation of the chemical, colloidal and biological properties of multicharged silica nanoparticles (SiO2NPs) obtained from its surface functionalization with zwitterionic groups, bifunctional structures and Ionic Liquids (IL). These groups have both positive and negative charges and total neutral charge, as well as have shown to be biocompatible and reduce nonspecific adsorption of proteins by structuring the water molecules around them. However, the few papers reported on this matter (or none for IL) have not simultaneously investigated the physicochemical and biological aspects of multicharged nanoparticles, which will regulate the physiological behavior of these nanostructures and their fate in vivo. Thus, multicharged structures will be synthesized and coupled on SiO2NPs surface, aiming understand their effect on the colloidal stability and nonspecific adsorption of biomolecules in physiological media simulated with bivalent ions and in fetal bovine serum. Subsequently, the in vitro behavior of nanostructures will be evaluated by cellular viability assays and hemocompatibility, and the cellular uptake of multicharged nanoparticles will be investigated by microscopy techniques. As a result of this study, it will be possible to create efficient methods of development, evaluation and application of nanoparticles for Nanomedicine. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, LARISSA FERNANDA; PICCO, AGUSTIN SILVIO; GALDINO, FLAVIA ELISA; CALUMBY ALBUQUERQUE, LINDOMAR JOSE; BERRET, JEAN-FRANCOIS; CARDOSO, MATEUS BORBA. Nanoparticle-Protein Interaction: Demystifying the Correlation between Protein Corona and Aggregation Phenomena. ACS APPLIED MATERIALS & INTERFACES, v. 14, n. 25, p. 11-pg., 2022-06-13. (17/02145-7, 18/09555-9, 21/12071-6, 20/00767-3, 14/21910-8, 19/24894-7, 15/25406-5)

DA CRUZ SCHNEID, ANDRESSA; ALBUQUERQUE, LINDOMAR JOSE CALUMBY; MONDO, GABRIELA BORBA; CEOLIN, MARCELO; PICCO, AGUSTIN SILVIO; CARDOSO, MATEUS BORBA. Colloidal stability and degradability of silica nanoparticles in biological fluids: a review. Journal of Sol-Gel Science and Technology, JAN 2022. (16/16905-0, 20/00767-3, 15/25406-5, 18/00763-8, 19/24894-7)