Inoculation of rizobacteria in a hydroponic system in nutrition, production and quality of American lettuce

• Grant number: 20/11621-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2021
• End date: February 28, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Marcelo Carvalho Minhoto Teixeira Filho
• Grantee: Carlos Eduardo da Silva Oliveira
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil

Abstract

Several researches have reported the multiple mechanisms of action and benefits of plant growth-promoting bacteria (BPCP) for Agriculture, however research in hydroponic systems is rare. Thus, it is believed that in a hydroponic system, inoculation with Azospirillum brasilense, Pseudomonas fluorescens and Bacillus subtilis can also increase the efficiency of use and accumulation of nutrients in the plant, reduce the concentrations of nitrate in the leaves and increase the lettuce fresh mass productivity. In addition, given the lack of information on this interaction of these bacteria (unprecedented research), there may be a synergistic effect between the use of A. brasilense, P. fluorescens and B. subtilis. Therefore, the objective of this research will be to evaluate the effect of inoculation with Azospirillum brasilense, Bacillus subtilis and Pseudomonas fluorescens in isolation and/or combined, in the nutrition, production and quality of American lettuce in NFT hydroponic cultivation. In addition to verifying the contribution of these BPCPs in agronomic biofortification with Fe and Zn, and reduction of nitrate concentration in lettuce leaves. For this, four experiments will be carried out in a randomized block design, with five replications. Each bench will receive a treatment. In all experiments, the inoculants of each BPCPs species will be applied in the nutrient solution at the time of transplanting the lettuce seedlings and at 10 days after transplanting, according to each experiment: experiment 1: inoculation with Azospirillum brasilense strains AbV5 and AbV6 (guarantee of 2x108 UFC mL-1), with doses of 0; 8; 16; 32 and 64 mL of the liquid inoculant for every 100 liters of nutrient solution; experiment 2: inoculation with Bacillus subtilis strain CCTB04 (1x108 UFC mL-1 guarantee), with doses of 0; 7.8; 15.6; 31.2 and 62.4 mL of the liquid inoculant for every 100 liters of nutrient solution; experiment 3: inoculation with Pseudomonas fluorescens strain CCTB03 (2x108 UFC mL-1 guarantee), with doses of 0; 8; 16; 32 and 64 mL of the liquid inoculant for every 100 liters of nutrient solution; experiment 4: will be carried out last, with the inoculation combined of BPCPs according to the optimal dose obtained for each inoculant in experiments 1, 2 and 3. The six treatments will be the following: a) control (without inoculation); b) inoculation with Azospirillum brasilense; c) Azospirillum brasilense + Bacillus subtilis; d) Azospirillum brasilense + Pseudomonas fluorescens; e) Bacillus subtilis + Pseudomonas fluorescens; f) Azospirillum brasilense + Bacillus subtilis + Pseudomonas fluorescens. Evaluations will be carried out: 1) fresh and dry lettuce production; 2) concentrations and accumulations of N, NO3-, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn in the shoots and roots of the lettuce; 3) calculation of the nutrient efficiency use; 4) physiological assessments with IRGA; 5) economic analysis of the production systems according to the treatment application. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARLOS EDUARDO DA SILVA OLIVEIRA; ARSHAD JALAL; JULIA REVOLTI OLIVEIRA; KAREN VICENTINI TAMBURI; MARCELO CARVALHO MINHOTO TEIXEIRA FILHO. Inoculação foliar de Azospirillum brasilense e Trichoderma harzianum em rúcula hidropônica melhoram os componentes produtivos e nutrição das plantas e reduzem o nitrato foliar. Pesqui. Agropecu. Trop., v. 52, 2022-09-02. (20/11621-0)

CARLOS E. DA S. OLIVEIRA; ISABELA M. B. GATO; VITORIA DE A. MOREIRA; ARSHAD JALAL; THAISSA J. S. S. OLIVEIRA; JULIA R. OLIVEIRA; GUILHERME C. FERNANDES; MARCELO C. M. TEIXEIRA FILHO. Métodos de inoculação de Azospirillum brasilense em alface e rúcula em sistema hidropônico. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 27, n. 9, p. 653-662, 2023-06-30. (20/11621-0)

MOREIRA, VITORIA DE ALMEIDA; OLIVEIRA, CARLOS EDUARDO DA SILVA; JALAL, ARSHAD; GATO, ISABELA MARTINS BUENO; OLIVEIRA, THAISSA JULYANNE SOARES SENA; BOLETA, GUILHERME HENRIQUE MARCANDALLI; GIOLO, VICTORIA MORAES; VITORIA, LETICIA SCHENAIDE; TAMBURI, KAREN VICENTINI; TEIXEIRA FILHO, MARCELO CARVALHO MINHOTO. Inoculation with Trichoderma harzianum and Azospirillum brasilense increases nutrition and yield of hydroponic lettuce. Archives of Microbiology, v. 204, n. 7, p. 12-pg., 2022-07-01. (20/11621-0)

DA SILVA OLIVEIRA, CARLOS EDUARDO; JALAL, ARSHAD; OLIVEIRA, JULIA REVOLTI; TAMBURI, KAREN VICENTINI; MINHOTO TEIXEIRA FILHO, MARCELO CARVALHO. Leaf inoculation of Azospirillum brasilense and Trichoderma harzianum in hydroponic arugula improve productive components and plant nutrition and reduce leaf nitrate. PESQUISA AGROPECUARIA TROPICAL, v. 52, p. 11-pg., 2022-01-01. (20/11621-0)

GATO, ISABELA MARTINS BUENO; DA SILVA OLIVEIRA, CARLOS EDUARDO; OLIVEIRA, THAISSA JULYANNE SOARES SENA; JALAL, ARSHAD; DE ALMEIDA MOREIRA, VITORIA; GIOLO, VICTORIA MORAES; VITORIA, LETICIA SCHENAIDE; DE LIMA, BRUNO HORSCHUT; VARGAS, PABLO FORLAN; FILHO, MARCELO CARVALHO MINHOTO TEIXEIRA. Nutrition and yield of hydroponic arugula under inoculation of beneficial microorganisms. HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, v. N/A, p. 16-pg., 2023-01-20. (20/11621-0)

OLIVEIRA, THAISSA JULYANNE SOARES SENA; OLIVEIRA, CARLOS EDUARDO DA SILVA; JALAL, ARSHAD; GATO, ISABELA MARTINS BUENO; RAUF, KAMRAN; MOREIRA, VITORIA DE ALMEIDA; DE LIMA, BRUNO HORSCHUT; VITORIA, LETICIA SCHENAIDE; GIOLO, VICTORIA MORAES; TEIXEIRA FILHO, MARCELO CARVALHO MINHOTO. Inoculation reduces nitrate accumulation and increases growth and nutrient accumulation in hydroponic arugula. Scientia Horticulturae, v. 320, p. 10-pg., 2023-06-07. (20/11621-0)

OLIVEIRA, CARLOS E. DA S.; GATO, ISABELA M. B.; MOREIRA, VITORIA DE A.; JALAL, ARSHAD; OLIVEIRA, THAISSA J. S. S.; OLIVEIRA, JULIA R.; FERNANDES, GUILHERME C.; TEIXEIRA FILHO, MARCELO C. M.. Inoculation methods of Azospirillum brasilense in lettuce and arugula in the hydroponic system1. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 27, n. 9, p. 10-pg., 2023-09-01. (20/11621-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Carlos Eduardo da Silva. Inoculation of rhizobacteria in a hydroponic system in the nutrition, production and quality of american lettuce. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira Ilha Solteira.