Effects of physical exercise on KLF15 protein and regulation of hepatic gluconeogenesis in hyperglycemic mice

• Grant number: 20/14320-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2021
• End date: September 01, 2023
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: José Rodrigo Pauli
• Grantee: Renan Fudoli Lins Vieira
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated scholarship(s): 22/02596-7 - Influence of age and diabesity on bedrest/microgravity-induced functional decline and upon recovery in mice, BE.EP.MS

Abstract

Adverse effects associated with antidiabetic drugs expose an increasing need for alternatives to conventional diabetes treatment and its complications. An efficient strategy is regular physical exercise, which has the potential to act at the origin of the disease. In this sense, our research aims to elucidate a new mechanism involved in hyperglycemia, with an action on transcription factor KLF15 (Kruppel like factor 15) that is a mediator of hepatic gluconeogenesis. For this purpose, experiments will be carried out on animals with hyperglycemia, knockout for GLUT4 (Slc2a4 +/-), this mice will be submitted to aerobic physical training protocol (running on a treadmill, five days, for 60 minutes, with intensity of 60% of maximum power and endurance). To confirm whether elevated glucose levels can induce an increase in KLF15 expression, an additional experiment will be carried out with liver cells treated with glucose, palmitate and insulin and also KLF15 overexpression and knockout assays, which will provide important insights into the behavior of KLF15 under these conditions and the relevance of this protein in the process of gluconeogenesis. The animals will be euthanized at 16h after the last training session, where the liver tissue will be extracted for analysis by RT-qPCR and Western Blot. It is hoped that the results obtained from this study may help to elucidate how physical exercise can impact the KLF15 pathway and the control of gluconeogenesis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AZEVEDO MACEDO, ANA PAULA; CALHEIROS ANTUNES, GABRIEL; LINS VIEIRA, RENAN FUDOLI; DE LIMA, ROBSON DAMASCENO. Cross-talk muscle and brown adipose tissue: Voluntary physical activity, aerobic training, time and temperature. JOURNAL OF PHYSIOLOGY-LONDON, v. 600, n. 17, p. 2-pg., 2022-08-11. (21/13847-8, 20/15679-2, 20/14320-0)