Purification and encapsulation of bacteriocins from probiotic bacteria for applications in animal protein production systems

• Grant number: 20/14144-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2021
• End date: May 15, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ricardo Pinheiro de Souza Oliveira
• Grantee: Nathalia Vieira Porphirio Veríssimo
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems, AP.TEM

Abstract

The growing concern with zoonotic diseases and multi-resistant microorganisms has driven the search for more sustainable alternatives than broad-spectrum antibiotics for the control of diseases in livestock. In this context, bacteriocins (antimicrobial peptides) are an excellent alternative for more rational management of zoonoses, due to their versatility in the control of different classes of microorganisms and their selective response to several cell targets. However, for the application of these substances in veterinary, it is necessary to reach certain standards of purity and formulation that guarantee their safety and effectiveness. As a consequence, a great obstacle for the commercial application of bacteriocins is to guarantee their purity and stability while maintaining competitive prices for this market. This is particularly challenging considering that the traditional techniques used for the purification and formulation of biomedicines were developed focusing on the pharmaceutical industry, which has requirements and costs well above those practiced in Veterinary Medicine. In this sense, this project aims to develop new sustainable and low-cost methodologies for purification and encapsulation of bacteriocins for use in animal production systems. As purification platforms, liquid-liquid extraction systems compatible with the biomolecules of this study will be applied, such as aqueous biphasic systems. For encapsulation, different polymeric systems formed by natural and synthetic polymers biocompatible with animals will be proposed. Finally, the best formulations will be evaluated in dynamic gastrointestinal systems for swine and birds, to determine products with the best veterinary potential. By developing new sustainable and inexpensive technologies for the production of veterinary probiotics, the main objective of the project is to reduce the risks related to zoonoses and increasing microbial resistance present in animal husbandry, aiming to maintain the position of Brazil as one of the world's largest producer and exporter in the livestock sector. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

V. VERISSIMO, NATHALIA; SAPONI, CAROLINA F.; RYAN, TIMOTHY M.; GREAVES, TAMAR L.; PEREIRA, JORGE F. B.. Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity. GREEN CHEMICAL ENGINEERING, v. 2, n. 4, p. 11-pg., 2021-12-01. (18/06576-5, 18/25511-1, 14/16424-7, 20/14144-8, 18/50009-8, 14/19793-3, 18/01858-2, 18/20833-0, 16/07529-5)

VERISSIMO, NATHALIA VIEIRA; MUSSAGY, CASSAMO USSEMANE; OSHIRO, ARIANE ALVES; NOBREGA MENDONCA, CARLOS MIGUEL; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; DE SOUZA OLIVEIRA, RICARDO PINHEIRO; BRANDAO PEREIRA, JORGE FERNANDO. From green to blue economy: Marine biorefineries for a sustainable ocean-based economy. GREEN CHEMISTRY, v. 23, n. 23, NOV 2021. (18/06908-8, 20/13271-6, 20/14144-8, 19/15493-9, 18/25511-1, 20/08655-0)

DE OLIVEIRA, RODRIGO CARDOSO; NOBREGA MENDONCA, CARLOS MIGUEL; VERISSIMO, NATHALIA VIEIRA; YOKOMIZO DE ALMEIDA, SONIA REGINA; CORREA, BENEDITO; WATANABE, II-SEI; DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid-producing Alternaria alternata on livestock feeds. Journal of Applied Microbiology, v. 133, n. 5, p. 10-pg., 2022-08-10. (19/16551-2, 20/13271-6, 18/25511-1, 20/14144-8, 20/10676-5, 19/02456-8)

VERISSIMO, NATHALIA VIEIRA; VICENTE, FILIPA A.; DE OLIVEIRA, RODRIGO CARDOSO; LIKOZAR, BLAZ; OLIVEIRA, RICARDO PINHEIRO DE SOUZA; PEREIRA, JORGE FERNANDO BRANDAO. Ionic liquids as protein stabilizers for biological and biomedical applications: A review. BIOTECHNOLOGY ADVANCES, v. 61, p. 29-pg., 2022-11-11. (18/25511-1, 19/15493-9, 20/14144-8, 20/10676-5, 18/06908-8)

HAN, QI; BROOMHALL, HAYDEN C.; VIEIRA VERISSIMO, NATHALIA; RYAN, TIMOTHY M.; DRUMMOND, CALUM J.; PEREIRA, JORGE F. B.; GREAVES, TAMAR L.. rotic Ionic Liquid Cation Alkyl Chain Length Effect on Lysozyme Structur. OLECULE, v. 27, n. 3, FEB 2022. (18/50009-8, 20/14144-8)