Genetic characterization of Salmonella spp. serotypes isolated from Poultry by whole genome sequencing

• Grant number: 20/07018-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2021
• End date: May 01, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Angelo Berchieri Junior
• Grantee: Valdinete Pereira Benevides
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 21/10285-9 - Whole-Genome Sequencing of multidrug-resistant Salmonella enterica strains isolated from commercial egg-laying farms in Brazil, BE.EP.DR

Abstract

Bacteria of Salmonella genus are pathogens that can infect animals and humans. Some Salmonella spp. serovars can settle down in poultry animals, in the digestive tract, and be disseminated without causing any apparent clinical signs. However, the presence of these bacteria can pose a risk to public health, making poultry products a potential source of infection to humans. Little is known about the factors associated with genetic diversity that could be contributing to the adaptation and persistence of Salmonella spp. in animal and human hosts. Furthermore, conventional methods of serotyping and identification of genetic determinants in Salmonella genus is laborious and can elapse for several days. This study proposes a comparative genomic analysis through the Whole Genome Sequencing (WGS) of paratyphoid Salmonella and identify possible genetic variations related to adaptation to the host in poultry animals. Additionally, it is proposed to characterize paratyphoid isolates, with identification of the genetic profiles of antimicrobial resistance, virulence and pathogenicity associated with isolates from humans and poultry. (AU)