Whole-genome sequencing approach to study multidrug-resistant Salmonella enterica isolates from Brazilian slaughterhouse

• Grant number: 17/15967-5
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 01, 2017
• Effective date (End): September 30, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology
• Principal Investigator: Mariza Landgraf
• Grantee: Daniel Farias Marinho Do Monte
• Supervisor abroad: Paula J. Fedorka-Cray
• Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Local de pesquisa : North Carolina State University (NC State), United States
• Associated to the scholarship: 16/03044-7 - Genetic diversity, virulence genes and antimicrobial resistance of Salmonella spp. isolated from poultry slaughterhouse, BP.DR

Abstract

The characterization of foodborne pathogen has changed over the years, although traditional typing systems based on phenotypic characteristics, such as antibiogram, biotype and serotype are still widely used. The advent of molecular biology and the use of techniques based on DNA analysis have revolutionized the field of epidemiology and pathogen characterization and whole genome sequencing (WGS) allows scientists and industries generate dataset quickly to solve problems. Bioinformatics can be used as a useful tool in the epidemiological investigation of Salmonella distributed in poultry production chain and this pathogen can now be examined in silico for the presence of antimicrobial resistance (AMR) and virulence genes immediately after sequencing, while traditional AMR testing is time-consuming and expensive. Considering this aspects, the present project aims to investigate the antimicrobial resistance, virulence and genotypic diversity of Salmonella serotypes important in Brazilian chicken production applying whole genome sequencing approaches. (AU)