Analysis of pro and anti-inflammatory adipocines in the different fat deposits of rats with metabolic syndrome

• Grant number: 21/01069-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2021
• End date: December 31, 2023
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Gabriela Souza Barbosa
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 22/04867-8 - Characterization of adipokines, energy expenditure and vascular phenotype in female and male mice with metabolic syndrome, BE.EP.IC

Abstract

The current nutritional transition process dded to a sedentary lifestyle has been associated with the remodeling and expansion of adipose tissue, that is, obesity. The various substances produced by this tissue are called adipokines, and include hormonesand other proteins such as cytokines, in this case called adipocytokines, among which areinterleukin-6 (IL-6), tumor necrosis factor alpha (TNF-±), and the monocytechemoattracting protein (MCP-1) and interleukin-10. In the body, we have several fatdeposits, and studies report that the accumulation of fat in a given location can increasethe inflammatory state, contributing to the evolution of the metabolic syndrome, which isalready recognized as a worldwide public health problem. That said, there may be adifference in the expression of anti and pro-inflammatory cytokines between differentdeposits of adipose tissue, it is important to characterize the profile of the releasedadipokines to facilitate the understanding of the role of different fat deposits in thepathophysiology of the metabolic syndrome. The objective of the work is to evaluate theconcentration of anti and pro-inflammatory adipokines in the different fat deposits ofWistar rats with metabolic syndrome. Male Wistar rats (n = 24) with 180 grams will beused which will be randomly distributed in two groups (n = 12 animals/group) to receivecontrol ration + water (control diet [DC]) or ration rich in simple carbohydrates and fat +drinking water plus 25% sucrose (High Sugar-Fat Diet [HSF]), composing the controlgroups (C) and HSF, respectively. Animals will receive this treatment throughout the 30-week trial period. Dietary intake and caloric intake will be controlled daily; caloric intakewill be determined by the product of consumption and the energy content of the diets. Tomonitor the animal's development, the weight will be measured weekly. At the end of theexperiment, systolic blood pressure will be measured by the indirect method of tailplestimography. In plasma, the levels of glucose, insulin, triglycerides, HDL and insulinresistance will be measured by calculating the HOMA-IR (Homeostasis ModelAssessment - Insulin Resistance). In the fat, visceral, epididymal, retroperitoneal andsubcutaneous deposits, the levels of cytokines IL-6, TNF-±, MCP-1, IL-10 andadiponectin will be measured. The adiposity index will also be carried out. The data willbe presented by descriptive measures of position and variability and analyzed by specific comparison tests.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, GABRIELA S.; COSTA, RAFAEL MENEZES; AWATA, WANESSA M. C.; SINGH, SHUBHNITA; ALVES, JULIANO, V; BRUDER-NASCIMENTO, ARIANE; CORREA, CAMILA R.; BRUDER-NASCIMENTO, THIAGO. Suppressed vascular Rho-kinase activation is a protective cardiovascular mechanism in obese female mice. BIOSCIENCE REPORTS, v. 43, n. 7, p. 13-pg., 2023-07-01. (21/01069-0)