Development of a three-dimensional polymeric structure to evaluate the effect of antimicrobials on the interaction of Aspergillus fumigatus

• Grant number: 20/11486-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2021
• End date: February 28, 2023
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Kelly Ishida
• Grantee: Bruna Nakanishi Fortes
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Aspergillus fumigatus infections are frequent in immunocompromised individuals and rely on biofilm formation that is associated with increased tolerance to antimicrobials and reinfections. A three-dimensional polymeric model forming a scaffold covered by lung cells can mimic the alveolar microenvironment in which this pathogen is found and thus become an important model for studying host-pathogen interaction as well as treatment strategies. Thus, this project aims to standardize the three-dimensional scaffold model formed by a hydroxyethyl-alginate-gelatin (HAG) cryogel with pulmonary epithelial cells and to evaluate the effect of antifungals on A. fumigatus infection. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

FORTES, BRUNA NAKANISHI; SCHEUNEMANN, GABY; MELO, ANALY SALLES DE AZEVEDO; ISHIDA, KELLY. Caspofungin alone or combined with polymyxin B are effective against mixed biofilm of Aspergillus fumigatus and carbapenem-resistant Pseudomonas aeruginosa. Research in Microbiology, v. 174, n. 1-2, p. 9-pg., 2023-01-01. (17/26076-4, 17/19374-9, 20/11486-5)

FORTES, BRUNA NAKANISHI; WIRTH, FERNANDA; DOS SANTOS, ALINE MARTINS; CHORILLI, MARLUS; FREITAS, VANESSA MORAIS; FARIAS, JENNIFER; CHAMBERGO, FELIPE S.; DANTAS, VIVIANE ABREU NUNES C.; ISHIDA, KELLY. Three-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatment. FUTURE MICROBIOLOGY, v. N/A, p. 14-pg., 2024-07-11. (20/11486-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FORTES, Bruna Nakanishi. Development of a three-dimensional polymeric structure to evaluate the effects of antifungals in the interaction with Aspergillus fumigatus. 2023. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.