Development of a three-dimensional polymeric structure to evaluate the effect of antimicrobials on the interaction of Aspergillus fumigatus

• Grant number: 20/11486-5
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2021
• Effective date (End): February 28, 2023
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Kelly Ishida
• Grantee: Bruna Nakanishi Fortes
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Aspergillus fumigatus infections are frequent in immunocompromised individuals and rely on biofilm formation that is associated with increased tolerance to antimicrobials and reinfections. A three-dimensional polymeric model forming a scaffold covered by lung cells can mimic the alveolar microenvironment in which this pathogen is found and thus become an important model for studying host-pathogen interaction as well as treatment strategies. Thus, this project aims to standardize the three-dimensional scaffold model formed by a hydroxyethyl-alginate-gelatin (HAG) cryogel with pulmonary epithelial cells and to evaluate the effect of antifungals on A. fumigatus infection. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FORTES, BRUNA NAKANISHI; SCHEUNEMANN, GABY; MELO, ANALY SALLES DE AZEVEDO; ISHIDA, KELLY. Caspofungin alone or combined with polymyxin B are effective against mixed biofilm of Aspergillus fumigatus and carbapenem-resistant Pseudomonas aeruginosa. Research in Microbiology, v. 174, n. 1-2, p. 9-pg., 2023-01-01. (17/26076-4, 17/19374-9, 20/11486-5)