Effects of cholinergic stimulation on inflammatory cell migration after myocardial infarction in SHR model

• Grant number: 20/14410-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2021
• Effective date (End): December 31, 2021
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Fernanda Marciano Consolim-Colombo
• Grantee: Manuella da Silva Teixeira
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

Cardiovascular diseases lead the top of the diseases with the highest mortality rate in the world, with myocardial infarction (MI) standing out as one of them. The immune system along with the autonomic nervous system plays an important role in the initiation and maintenance of hypertension and contributes to the development of cardiovascular pathology. The modulation of the immune response via the vagus nerve, called the Cholinergic Anti-inflammatory pathway, is shown as a therapeutic potential in face of inflammatory response. Previous studies by our group have shown that cholinergic stimulation by pyridostigmine bromide (PB, acetylcholinesterase inhibitor) decreases the inflammatory response in different experimental models. Furthermore, it increases parasympathetic activity, modulates immune cells in the myocardium and improves ventricular function parameters. However, little is known about the impact of cholinergic stimulation on the modulation of inflammatory cells in cardiac tissue after MI in spontaneously hypertensive rats (SHR).Objective: To evaluate the effects of cholinergic stimulation on migration of inflammatory cells after myocardial infaction in SHR model. Methods: We will use adult male SHR rats to be divided into 3 groups: SHAM (simulated operation), AMI (infarcted) and AMI + BP7 (infarcted + treated with pyridostigmine 40mg / Kg / day, for 7 days). Pyridostigmine will be administered by gavage, starting immediately 1 hour after AMI or simulated operation 1x day. Euthanasia will be performed on the 30th day, followed by removal of biological material for histological, immunohistochemistry and gene expression analyzes. (AU)