Health challenge effect on the heat production partitioning and energy utilization in broiler chicks

• Grant number: 21/08142-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: August 01, 2021
• End date: August 31, 2024
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Nilva Kazue Sakomura
• Grantee: Rosiane de Souza Camargos
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 19/26575-6 - Net energy system for poultry, AP.TEM

Abstract

It is important to accurately energy requirements expression of birds. Therefore, it is essential to know the mechanism of action of an animal in the face of a disease. The animal's metabolism is altered, modulating to readjust the requirements of the animal in that condition. The objective is to evaluate the energy expenditure of birds, as well as to describe the partition of heat production and energy use in conditions of health challenges. In the first test, 375-day-old chicks, Cobb 500 males, will be distributed in a completely randomized design in 3 treatments (T1 - birds not challenged with ad libitum feed intake; T2 - birds challenged (Eimeria maxima and Clostridium) and T3 - birds not challenged with feed intake equivalent to the previous day of the ad libitum feed intake of the challenged birds) with five repetitions of 25 birds each. The challenge will be performed on the 14th day of age, through inoculation of Eimeria maxima (7x103 oocysts/ml) and on days 18, 19 and 20 inoculation of Clostridium perfringens (2.5x106UFC). Parallel to this experiment, 60 birds submitted to treatments: T1 - challenged (Eimeria and Clostridium) and T2 - pair feeding (2 treatments x 3 repetitions x 10 birds) will be housed in respirometric chambers (6 chambers) from 14 to 23-days-old and gas exchange parameters will be measured to calculate the total heat production. In the third trial, the birds will be challenged with Lipopolysaccharide (LPS). 21-day-old chicks, Cobb 500 males, will be used, distributed in a completely randomized design in 5 treatments (T1 - birds not challenged with ad libitum feeding; T2 - challenge 1 (10 µg de LPS/kg - E. coli, 0127:B8; Sigma-Aldrich Brazil); T3 - not challenged with feed offered equivalent to the T2 bird's feed intake; T4 - Challenge 2 (100 µg de LPS/kg - E. coli, 0127:B8; Sigma-Aldrich Brazil); T5 - not challenged with feed offered equivalent to the T4 bird's feed intake. Each treatment with four repetitions of one bird per each. Performance, nutrient deposition in the body, digestibility, heat production, internal body temperature, immunological and molecular parameters will be determined. (AU)