Fabrication of 3D microenvironments for the development of microorganisms

• Grant number: 20/15736-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2021
• End date: February 28, 2023
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Cleber Renato Mendonça
• Grantee: Jonathas Queiroz Ribeiro Moraes
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 18/11283-7 - Nonlinear photonics: spectroscopy and advanced processing of materials, AP.TEM

Abstract

Probiotics are microorganisms that bring benefits to their host and may have positive immunologic effects by stimulating the increase of beneficial bacteria of the gastrointestinal microbiota. Some studies have been showing that probiotics can lessen symptoms of various medical conditions, such as Alzheimer disease, tooth decay, diabetes, and others. Therefore, it is important to determine fundamental aspects about the formation of probiotic biofilms, in order to create more efficient delivery mechanisms. Hence, this project aims to fabricate biocompatible tridimensional platforms using the two-photon polymerization (2PP) technique in order to study the growth of biological films of Lactobacillus sp., a bacterium largely utilized in commercial probiotics. The microstructures will be made of acrylic resins via ultra-short laser pulses. After, the biocompatibility of these frameworks will be studied and, lastly, the biofilm development as well.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MORAES, Jonathas Queiroz Ribeiro. Fabrication of 3D microenvironments for the development of microorganisms. 2023. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.