Assessment by RT-PCR of circulant tumor cells in the peripheral blood of patients with gastric Cancer

• Grant number: 21/05357-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2021
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Ulysses Ribeiro Júnior
• Grantee: Gabriel da Silva Kawakami
• Host Institution: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). Coordenadoria de Serviços de Saúde (CSS). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Gastric Cancer (GC) is the 5th most common cancer in the world with an estimated incidence of 21,230 new cases in 2020 in Brazil. Adenocarcinoma is the most common histological type of GC and its diagnosis is made by means of upper gastrointestinal endoscopy (EDA), followed by staging by the TNM classification. In addition, laparoscopy can be adopted because it has greater accuracy in detecting metastases, which determines a change in treatment and is able to identify patients at high risk of recurrence, associated with lower survival rates. The most commonly adopted treatment is total or partial gastrectomy with D1 or D2 lymphadenectomy. Because hematological recurrence is the second most frequent cause of failure in the treatment of GC, the detection of free circulating tumor cells (CTLC) in peripheral blood, using the RT-PCR method, can be a relevant tool to predict the recurrence of metastases and determining the patient's prognosis. However, no consensus has been reached regarding the correlation between the presence of CTLC in peripheral blood and its impact on patient survival. In this context, the present study aims to evaluate the presence of CTLC, using the qRT-PCR technique with the use of CK20, TFF1, and MUC1 markers in peripheral blood samples from patients with GC, in addition to verifying the association of the presence of CTLC with clinicopathological parameters, recurrence, and overall patient survival. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GABRIEL DA SILVA KAWAKAMI; MARINA ALESSANDRA PEREIRA; MÁRCIA SALDANHA KUBRUSLY; ALEXIS GERMÁN MURILLO CARRASCO; MARCUS FERNANDO KODAMA PERTILLE RAMOS; ULYSSES RIBEIRO JÚNIOR. AVALIAÇÃO POR RT-PCR DE EXPRESSÃO DE MARCADORES TUMORAIS EM SANGUE PERIFÉRICO DE PACIENTES COM CÂNCER GÁSTRICO. ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY, v. 36, 2024-02-05. (21/05357-0)