Proteomic and phosphoproteomic analyses of the prostate of the senescent rats exposed to a phthalate mixture during gestation and lactation

• Grant number: 21/06617-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 30, 2022
• End date: June 29, 2023
• Field of knowledge: Biological Sciences - Morphology - Embryology
• Principal Investigator: Wellerson Rodrigo Scarano
• Grantee: Ariana Musa de Aquino
• Supervisor: Joern Dengjel
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Université de Fribourg, Switzerland
• Associated to the scholarship: 19/13823-1 - Identification of oncogenic targets in the prostate of rats exposed to fatalate mixtures during perinatal development: transgerational study, BP.DR

Abstract

Phthalates represent a class of molecules detected in different concentrations in breast milk, in the urine of pregnant women, and in the human and rodent placenta. Previous studies have shown that perinatal exposure to isolated phthalates increased susceptibility to prostatic carcinogenesis. Thus, the study seeks to investigate the OMICS profile on ventral prostate (VP) of senescent rats exposed to a mixture of phthalates in different concentrations, focusing on possible oncogenic of animals exposed in the perinatal period (F1). For this, Sprague-Dawley pregnant rat were exposed to different concentrations of phthalates mixture for simulated the low exposure (T1:20µg/kg/day), environmentally relevant; high exposure (T2:200mg/kg/day), occupational exposure and not directly exposure (C: control; vehicle). The pregnant females received treatment by orally gavage from gestational day 10 (GD10) to postnatal day 21 (PND21). The F1 male rats were euthanized in three times: PND22, PND120 and PND540. Exposed animals that were not euthanized (F1) were mated with unexposed animals and their offspring (F2) were euthanized on PND22. The VP from F1 and F2 animals were collected for proteomic analysis in both generations, to trace possible molecular mechanisms related to the effects of treatments. Specific analysis to evaluate senescent prostate (PND540; F1) to obtain OMICs data will be made at the Swiss host lab. Additionally, we intend to compare the results with previous data from F1 and F2 for in silico analysis. (AU)