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Evaluation of different methods superficial treatment of dentin in non-carious cervical lesions using scanning electron microscopy and optical profilometry

Grant number: 21/03874-8
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2021
Effective date (End): July 31, 2022
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Ana Cecília Corrêa Aranha
Grantee:Maria Eduarda Peres
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The aim of this study will be to evaluate, in vitro, the effect of different treatments applied to dentin, previously to an adhesive system for adhesive restoration. The surface roughness and wettability of the treatments will be observed in the bond strength of cervical restorations in composite resin, as well as the morphological changes of these treated surfaces. Fifty dentin specimens (human molars) will be obtained, approximately 2 mm thick, which will be subjected to an erosive and abrasive challenge, to simulate a non-carious cervical lesion. Afterward, they will be submitted to different surface treatments: aspiration, blasting with aluminum oxide, aspiration followed by blasting with aluminum oxide, Er: YAG laser, and without treatment, for control, using two types of self-etching adhesives, of two steps and universal. Then the specimens will be prepared for scanning electron microscopy (SEM), to qualitatively check the dentin surface after erosive-abrasive cycling and post-treatments, for 3D profiling, to analyze the surface roughness, the wetness of the treated specimens will be evaluated. through the analysis of the contact angle goniometer. The data obtained will be analyzed by statistical analysis, using the Tukey test (± = 0.05). (AU)

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