Characterization of the role of Nop17, r2tp-complex member, in the cytosolic iron-sulfur protein assembly (CIA) system in Saccharomyces cerevisiae

• Grant number: 21/06497-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: March 14, 2022
• End date: March 13, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Carla Columbano de Oliveira
• Grantee: Felipe Aguilar Carvalho
• Supervisor: Roland Lill
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Philipps-Universität Marburg, Germany
• Associated to the scholarship: 19/00527-5 - Characterization of the role of Nop17, R2TP complex-member, in the cytosolic Fe-S protein assembly (CIA) system in Saccharomyces cerevisiae, BP.DD

Abstract

In vivo Fe-S cluster assembly into target apo-proteins requires a complex and conserved biosynthetic machinery. In non-green eukaryotes two systems are dedicated to mature Fe-S cluster containing proteins, known as ISC (Iron-Sulfur Cluster Assembly) and CIA (Cytosolic Iron-Sulfur Protein Assembly) systems. The ISC system takes place inside mitochondria and is responsible for the direct maturation of all Fe-S cluster containing proteins within the organelle and for the indirect maturation of all extra-mitochondrial Fe-S cluster containing proteins, through the export of an unknown sulfur-containing (X-S) compound. The CIA system occurs in the cytosol and rely on the X-S compound to mature all cytosolic and nuclear Fe-S cluster containing proteins. Our laboratory has identified an interaction between the CIA protein Dre2 and the R2TP cochaperone complex member Pih1/Nop17; moreover, other works showed the interaction between Hsp90 and other CIA proteins. The R2TP-Hsp90 complex matures a diverse range of client proteins, such as box C/D snoRNPs and RNA polymerase II. The interaction between either Nop17 or Hsp90 and CIA proteins suggest the participation of the R2TP-Hsp90 complex in the CIA system. Previous experiments in our laboratory have also indicated the presence of a [4Fe-4S] cluster in Nop17. In this project, we propose the characterization of the role of the R2TP-complex in the CIA system, as well as the structural characterization of the Fe-S cluster of Nop17. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, FELIPE A.; MUEHLENHOFF, ULRICH; BRAYMER, JOSEPH J.; ROOT, VASILIJ; STUEMPFIG, MARTIN; OLIVEIRA, CARLA C.; LILL, ROLAND. Hsp90 and metal-binding J-protein family chaperones are not critically involved in cellular iron-sulfur protein assembly and iron regulation in yeast. FEBS Letters, v. 597, n. 13, p. 15-pg., 2023-03-30. (19/00527-5, 20/00901-1, 21/06497-0)