Speciation, bioaccumulation and biotransformation of arsenic by cyanobacteria (Arthrospira sp. and Anabaenopsis sp.) from the saline-alkaline lakes of the Pantanal of Nhecolândia

• Grant number: 21/05927-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2021
• End date: February 29, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Anne Helene Fostier
• Grantee: Marianna Alves Cancian
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/14227-5 - Climatic changes and environmental impacts in wetlands of Pantanal region (Brazil): quantification, control factors and long term temporal modeling, AP.PFPMCG.TEM
• Associated scholarship(s): 22/12982-1 - Interactions between arsenic species and natural organic matter of the saline-alkaline lakes of the Pantanal of Nhecolândia, BE.EP.MS

Abstract

Results obtained in the thematic project FAPESP 2016/14227-5 demonstrated that: 1) the waters of the alkaline lakes of the Pantanal of Nhecolândia systematically present high concentrations of dissolved As (up to 3000 µg L-1); 2) despite the extreme conditions, these lakes are home to an abundant microbial community; 3) higher dissolved As concentrations are generally found in lakes where the highest autotrophic activity occurs (green lakes) and which consequently have higher organic matter production; 4) the phytoplanktonic organisms in these lakes have a low bioconcentration factor regarding arsenic. This last fact may be due to the ability of microorganisms to tolerate and/or metabolize As, or even due to various processes occurring in the water column that limit As bioavailability (e.g. complexation with organic matter). In any case, phytoplankton seems to play an essential role in the cycle of this element, as it can influence its speciation and bioavailability. This project is part of this framework and aims to elucidate the role of two species of cyanobacteria (Limnospira sp. and Anabaenopsis sp.) in As speciation, bioaccumulation and biotransformation and thus better understand their role in the As biogeochemical cycle in this ecosystem. The two cyanobacteria, previously isolated at the CENA/USP Cellular and Molecular Biology Laboratory from phytoplankton samples collected in alkaline lakes, will be cultivated in this laboratory in a medium enriched with As(V) or As(III). The concentrations of total As and of each As(V), As(III), MMA and DMA species in the different types of samples (culture medium and cells) will be determined by High Pressure Liquid Chromatography (HPLC) coupled with on-line UV irradiation, Hydride Generation and Atomic Fluorescence Spectrometry (HPLC-UV-HG-AFS). Bioconcentration factors will be calculated as the ratio betweenf As concentration in the cells and in the culture medium.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA, JOSE LUCAS MARTINS; STEFFLER, DEBORA APARECIDA; HERNANDEZ, AMAURIS HECHAVARRIA; COSTA, JULIANA DOS SANTOS; PELLEGRINETTI, THIERRY ALEXANDRE; DE JESUS, ERICK CHRISTIAN RODRIGUES; CANCIAN, MARIANNA; FIORE, MARLI FATIMA; REZENDE-FILHO, ARY T.; SUSSULINI, ALESSANDRA; et al. Bioaccumulation and speciation of arsenic in plankton from tropical soda lakes along a salinity gradient. Science of The Total Environment, v. 895, p. 11-pg., 2023-07-03. (17/12644-0, 19/18124-4, 16/14227-5, 17/23301-7, 21/05927-1, 21/05683-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CANCIAN, Marianna Alves. Cyanobacteria and natural organic matter associated with the biogeochemical cycle of arsenic in the saline-alkaline lakes of the Nhecolândia Pantanal. 2024. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.