The phytochemical study, development, and validation of an analytical method by HPLC-DAD for analysis of the major constituents of Petiveria alliacea

• Grant number: 21/05175-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2021
• End date: December 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Patricia Mendonça Pauletti
• Grantee: Ina Blaine de Alvarenga Pereira
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Petiveira alliacea L. is a perennial shrub from the Phytolaccaceae family, it is a medicinal plant found in Latin America, which grows in tropical regions of the South and Center continent, the Caribbean, and Africa. This species is popularly known as "guinea", "mucuracaá" and "pipi", being used in folk medicine in antirheumatic treatment, as an analgesic, and for the treatment of respiratory problems. Pharmacological studies of this species have shown the therapeutic potential of P. alliaceae as an immunomodulator, analgesic, antimicrobial and anticancer. Our research group has recently been evaluating the antimicrobial properties of extract and fractions of P. alliaceae. This information, combined with the data described in the literature, and their use by the population corroborate the importance of the development and validation of an analytical method for the quality control of this medicinal plant. Thus, this project goals to carry out a phytochemical study aiming to isolate and identify the major components of the n-butanol fraction of the crude extract aerial parts, as well as to develop and validate an analytical method by High-performance liquid chromatography coupled with a diode array detector (HPLC-DAD), at least one of the isolated compounds will be used as standard, to be used in the quality control of this species and also contribute to the training of human resources in Franca region. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HENRIQUE, CAMILA YAMASITA; PEREIRA, INA BLAINE DE ALVARENGA; AFONSO, LAURA FERNANDES; BAROSELA, JOSE RICARDO; SILVA, SARAH CRISTINA ANDRADE; ALVES, OSVAINE JUNIOR ALVARENGA; CUNHA, WILSON ROBERTO; SILVA, MARCIO LUIS ANDRADE E; JANUARIO, ANA HELENA; LEONEL, ALEXANDRE HENRIQUE; et al. Flavonoid quantitative HPLC-DAD analysis of Petiveria alliacea. Journal of Liquid Chromatography & Related Technologies, v. 47, n. 16-20, p. 8-pg., 2024-06-27. (17/24860-0, 16/10313-4, 16/06260-2, 21/05175-0, 22/12597-0)