Copaifera oleoresins: development and validation of a HPLC-DAD analytical method and phytochemical study and in vitro antitumor evaluation of the main secondary metabolites obtained

• Grant number: 16/01232-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2016
• End date: April 30, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Sergio Ricardo Ambrosio
• Grantee: Luiza Junqueira Carneiro
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

Despite of the wide folk use of copaiba oil resin by the Brazilians since XIX century and several developed studies corroborating many of its ethno pharmacological uses, as well as its production being around 800 tons per year, there is a great need to fully determine the following parameters: phytochemical, pharmacological, toxicological and clinical studies, aiming to guarantee safety, efficacy and quality, which are mandatory for developing new phytomedicines. In this sense, the goal of this proposal is to undertake the chemical validation of the oleoresin of Copaifera multijuga, Copaifera pubiflora and Copaifera trapezifolia, to evaluate the antitumor activity (in vitro) of the major secondary metabolites isolated from this natural sources, as well as the development and validation of a HPLC-DAD analytical method to the main oleoresins commercialized in Brazil: C. multijuga, C. duckei, C. reticulate, C. pubiflora and C. paupera. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARNEIRO, LUIZA J.; BIANCHI, THAMIRES C.; DA SILVA, JONAS J. M.; OLIVEIRA, LARISSA C.; BORGES, CARLY H. G.; LEMES, DANIELI C.; BASTOS, JAIRO K.; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO R.. Development and Validation of a Rapid and Reliable RP-HPLC-PDA Method for the Quantification of Six Diterpenes in Copaifera duckei, Copaifera reticulata and Copaifera multijuga Oleoresins. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. 729-737, APR 2018. (16/01232-0, 14/03092-6, 11/13630-7)

CARNEIRO, LUIZA J.; BIANCHI, THAMIRES C.; DA SILVA, JONAS J. M.; OLIVEIRA, LARISSA C.; BORGES, CARLY H. G.; LEMES, DANIELI C.; BASTOS, JAIRO K.; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO R.. Development and Validation of a Rapid and Reliable RP-HPLC-PDA Method for the Quantification of Six Diterpenes in Copaifera duckei, Copaifera reticulata and Copaifera multijuga Oleoresins. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. 9-pg., 2018-04-01. (14/03092-6, 11/13630-7, 16/01232-0)

CARNEIRO, LUIZA J.; TASSO, THERCIUS O.; SANTOS, MARIO F. C.; GOULART, MIRIAN O.; DOS SANTOS, RAQUEL; BASTOS, JAIRO K.; DA SILVA, JONAS J. M.; CROTTI, ANTONIO E. M.; PARREIRA, RENATO L. T.; ORENHA, RENATO P.; et al. Copaifera multijuga, Copaifera pubiflora and Copaifera trapezifolia Oleoresins: Chemical Characterization and in vitro Cytotoxic Potential against Tumoral Cell Lines. Journal of the Brazilian Chemical Society, v. 31, n. 8, p. 1679-1689, AUG 2020. (16/01232-0, 11/13630-7)