Copaifera langsdorfii oleoresin: isolation of secondary metabolites, development and validation of analytical methodology by HPLC

• Grant number: 09/12796-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2010
• End date: February 29, 2012
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Rodrigo Cassio Sola Veneziani
• Grantee: Ariana Borges Souza
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Many plants are sources of active secondary metabolites that can be employed more directly as phytomedicines or in their isolated forms as drugs themselves, or even as semi-synthethic precursors of new drugs. In this sense, it is important to highlight that quality control and standardization of plants raw materials are a central issue in successful strategies that use natural products for the development of new drugs, as well as to ensure the quality of the already available ones. The Copaifera langsdorfii oleoresin is a natural product very valuable for pharmaceutical companies due to its antiinflammatory, analgesic and antimicrobial potential. In addition, its value is reinforced by its use in fragrance, cosmetic and varnishes industry. It is very common to find out in the market oleoresins whose origin is not well established. Due to this problem, analytical methodologies used for the qualitative and quantitative characterization of C. langsdorfii oleoresin play a key role in the standardization and quality insurance of this material. In this project, it is proposed the isolation of analytical standards and the development and validation of an analytical methodology by High Performance Liquid Chromatography (HPLC) in order to allow the direct analysis of this oleoresin, avoiding the chemical derivatization step, which is commonly found in the current used Gas Chromatography methods (GC). In this sense, a HPLC direct analysis of C. langsdorfii oleoresin will be more practical and fast than the GC approach.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, ARIANA BORGES; MOREIRA, MONIQUE RODRIGUES; GAMBETA BORGES, CARLY HENRIQUE; SIMAO, MARILIA RODRIGUES; BASTOS, JAIRO KENUPP; BARRETO DE SOUSA, JOAO PAULO; AMBROSIO, SERGIO RICARDO; SOLA VENEZIANI, RODRIGO CASSIO. Development and validation of a rapid RP-HPLC method for analysis of (-)-copalic acid in copaiba oleoresin. BIOMEDICAL CHROMATOGRAPHY, v. 27, n. 3, p. 280-283, MAR 2013. (11/13630-7, 09/12796-9)

SOUZA, ARIANA B.; DE SOUZA, MARIA G. M.; MOREIRA, MAISA A.; MOREIRA, MONIQUE R.; FURTADO, NIEGE A. J. C.; MARTINS, CARLOS H. G.; BASTOS, JAIRO K.; DOS SANTOS, RAQUEL A.; HELENO, VLADIMIR C. G.; AMBROSIO, SERGIO RICARDO; et al. Antimicrobial Evaluation of Diterpenes from Copaifera langsdorffii Oleoresin Against Periodontal Anaerobic Bacteria. Molecules, v. 16, n. 11, p. 9611-9619, 2011. (09/12796-9, 09/09438-3)