Development and validation of analytical methodology by HPLC for quantification of genic acid in cosmetic emulsion

• Grant number: 19/00172-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2019
• Effective date (End): July 31, 2020
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Marcos Antonio Corrêa
• Grantee: Mariana Carlomagno de Paula
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Gentilic acid (AGe) is a non-flavonoid phenolic compound derived from hydroxybenzoic acid. It has a carboxylic group attached to the aromatic ring, plus two hydroxyl groups attached at the 2 and 5 positions. AGe is an active metabolite of salicylic acid degradation, which has a broad spectrum of biological activity, including antioxidant, astringent, anti-inflammatory properties and even antirheumatic. For the study of this acid, will be developed and validated analytical methodology by HPLC. For the study of this acid, will be developed and validated an analytical methodology by HPLC. The validation of analytical methods is a process that through experimental studies ensures the reliability of the results obtained by these, and then is of extreme importance in assuring the quality of cosmetics. The project aims to develop and validate the analytical methodology by HPLC for the quantification of gentisic acid in cosmetic emulsion. An emulsified system oil-in-water (O/W) will be developed for the incorporation of the active substance, and to quantify it in this emulsion, an analytical method will be developed and validated by High Performance Liquid Chromatography.