Analysis of the metabolic changes in H9c2 myocytes during the differentiation

• Grant number: 21/08994-1
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2021
• Effective date (End): September 30, 2022
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal researcher: Aline Mara dos Santos
• Grantee: Flávia Oliveira Borges Pereira
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The heart is one of the most active organs in the human body and its functioning is directly connected to the maintenance of life. Diseases and conditions related to the poor functioning of this organ are among the main causes of death not only in Brazil but in the world; however, although the heart's low regenerative capacity is already known, its causes are not fully understood. It is known that during the differentiation process, metabolic changes occur in myocytes, concomitantly with the loss of their proliferative capacity, a phenomenon that impacts the regeneration of this organ. The present work aims to use techniques associated with metabolomics science to identify and analyze the metabolic alterations of cardiac myocytes during differentiation, in order to understand the metabolic processes involved in the loss of their proliferative capacity. For this, cardiomyoblasts of the H9c2 lineage will be cultured and induced to differentiate into cardiac myocytes; at different stages of this process, a collection of the endo and exometabolome will be made followed by analysis by GC-MS; at the end, the data obtained will be processed by software dedicated to the metabolomic analysis and statistical tools in order to differentiate the groups of samples (cardiomyoblasts and cardiomyocytes) and understand the metabolic changes that have occurred and their consequences for this cells.(AU)