Effect of global or conditional deletion (skeletal muscle) of the Nr1d1 gene on aging

• Grant number: 21/08693-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2021
• End date: July 20, 2026
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Ana Paula Pinto
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 19/11820-5 - Nr1d1 function on the aging-associated Sarcopenia, AP.TEM
• Associated scholarship(s): 22/16162-9 - The characterization of Rev-Erb-alpha on different metabolic conditions: from mice to humans, BE.EP.PD

Abstract

Rev-erb-alpha is an important physiological regulator of autophagy, mitochondrial content, oxidative function, and maintenance of skeletal muscle mass, making this protein a promising molecular target for the prevention and treatment of Sarcopenia. However, studies in the scientific literature were carried out in rodents with global Nr1d1 deletion, which makes it impossible to differentiate between systemic and local functions of rev-erb-alpha in skeletal muscle physiology. Thus, the application of experimental conditional knockout models is crucial for mechanistic advances regarding the role of the rev-erb-alpha protein in aging-associated Sarcopenia. Therefore, the general objective of this project will be to verify the role of the global or specific (skeletal muscle) deletion of Nr1d1 in the aging process. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARAFON, BRUNO B.; PINTO, ANA P.; ROPELLE, EDUARDO R.; DE MOURA, LEANDRO P.; CINTRA, DENNYS E.; PAULI, JOSE R.; DA SILVA, ADELINO S. R.. Muscle endoplasmic reticulum stress in exercise. ACTA PHYSIOLOGICA, v. 235, n. 1, p. 13-pg., 2022-02-21. (21/08693-1, 20/04269-8, 19/11820-5)

MARAFON, BRUNO BRIEDA; PINTO, ANA PAULA; DE SOUSA NETO, IVO VIEIRA; DA LUZ, CAROLINE MANTOVANI; PAULI, JOSE RODRIGO; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; SIMABUCO, FERNANDO MOREIRA; DE MOURA, LEANDRO PEREIRA; DE FREITAS, ELLEN CRISTINI; et al. The role of interleukin-10 in mitigating endoplasmic reticulum stress in aged mice through exercise. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v. 327, n. 3, p. 12-pg., 2024-09-01. (21/06291-3, 21/08693-1, 21/14233-3, 19/11820-5, 22/09100-7)

DA ROCHA, ALISSON L.; PINTO, ANA P.; BEDO, BRUNO L. S.; MORAIS, GUSTAVO P.; OLIVEIRA, LUCIANA C.; CAROLINO, RUITHER O. G.; PAULI, JOSE R.; SIMABUCO, FERNANDO M.; DE MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; et al. Exercise alters the circadian rhythm of REV-ERB-alpha and downregulates autophagy-related genes in peripheral and central tissues. SCIENTIFIC REPORTS, v. 12, n. 1, p. 13-pg., 2022-11-21. (21/08693-1, 19/17532-1, 17/25492-4, 19/11820-5, 17/19869-8, 17/12765-2)

PINTO, ANA P.; MUNOZ, VITOR R.; DA ROCHA, ALISSON L.; ROVINA, RAFAEL L.; FERRARI, GUSTAVO D.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. IL-6 deletion decreased REV-ERB alpha protein and influenced autophagy and mitochondrial markers in the skeletal muscle after acute exercise. FRONTIERS IN IMMUNOLOGY, v. 13, p. 13-pg., 2022-10-13. (21/06291-3, 21/08693-1, 19/11820-5, 17/12765-2, 19/21709-4, 21/08692-5, 20/13443-1, 18/14818-9)

FERREIRA, DRIELE C. DA SILVA; PINTO, ANA PAULA; NETO, IVO VIEIRA DE SOUSA. Limited impact of blood flow restriction on muscle quality of young individuals during prolonged unloading: Implications for therapeutics approaches. JOURNAL OF PHYSIOLOGY-LONDON, v. N/A, p. 3-pg., 2024-06-26. (23/02988-5, 21/08693-1, 21/14233-3, 22/16162-9)

NETO, IVO VIEIRA DE SOUSA; PINTO, ANA PAULA; ANDRADE, ROSANGELA VIEIRA DE; DE SOUZA, FABIANE HIRATSUKA VEIGA; DE SOUZA, PAULO EDUARDO NARCIZO; ASSIS, VICTORIA; TIBANA, RAMIRES ALSAMIR; NEVES, RODRIGO VANERSON PASSOS; ROSA, THIAGO SANTOS; PRESTES, JONATO; et al. Paternal exercise induces antioxidant defenses by α-Klotho/Keap1 pathways in the skeletal muscle of offspring exposed to a high fat-diet without changing telomere length. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 134, p. 15-pg., 2024-09-23. (21/06291-3, 21/08693-1, 21/08692-5, 21/14233-3, 19/11820-5)

RAMOS, ELIEZER LUCAS PIRES; NETO, IVO VIEIRA DE SOUSA; PINTO, ANA PAULA; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; DE FREITAS, ELLEN CRISTINI; MINEO, TIAGO WILSON PATRIARCA; DA SILVA, ADELINO SANCHEZ RAMOS. Mechanisms underlying the interplay between autophagy and the inflammasome in age-related diseases: Implications for exercise immunology. AGEING RESEARCH REVIEWS, v. 110, p. 12-pg., 2025-08-01. (21/14233-3, 23/16626-8, 21/08693-1, 19/13210-0, 19/11820-5)

PINTO, ANA P.; MUNOZ, VITOR R.; TAVARES, MARIA EDUARDA A.; DOS SANTOS, JONATHAS R.; REBELO, MACARIO A.; ALBERICI, LUCIANE C.; SIMABUCO, FERNANDO M.; TEIXEIRA, GIOVANA R.; PAULI, JOSE R.; DE MOURA, LEANDRO P.; et al. Combined physical exercise reverses the reduced expression of Bmal1 in the liver of aged mice. Life Sciences, v. 312, p. 12-pg., 2023-01-01. (21/08693-1, 21/08692-5, 20/13443-1, 21/06291-3, 19/11820-5)

DA MATA, GUSTAVO EDUARDO; BRICOLA, RAFAEL; RIBEIRO, DANIELLE NAVES; SIMABUCO, FERNANDO M.; PAULI, JOSE R.; DE FREITAS, ELLEN C.; ROPELLE, EDUARDO R.; DA SILVA, ADELINO S. R.; PINTO, ANA P.. Acute exercise modulates Trim63 and Bmal1 in the skeletal muscle of IL-10 knockout mice. CYTOKINE, v. 175, p. 9-pg., 2023-12-30. (21/06291-3, 21/08693-1, 19/11820-5)