Phase angle behavior and bioelectrical impedance vectors and their relation with indirect markers of eccentric muscle damage after ischemic preconditioning: a randomized placebo-controlled clinical trial

• Grant number: 21/11893-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: November 30, 2022
• Field of knowledge: Biological Sciences - Physiology - Physiology of Effort
• Principal Investigator: Franciele Marques Vanderlei
• Grantee: Julia Waszczuk Vendrame
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

Eccentric Exercise (EE) has been applied in rehabilitation and improvement of physical fitness due to its mechanical advantage and lower metabolic demand, however, EE can induce muscle damage, resulting in changes and reduction in muscle function. Therefore, there are some tools such as the concentration of creatine kinase (CK) and lactate dehydrogenase (LDH) that can be used as indirect markers of muscle damage caused by EE. Another tool that may be able to assess cellular health responses and cellular integrity is the bioelectrical impedance (BIA), therefore, it's important to look for alternatives to reduce this damage caused by stress. Hence, ischemic preconditioning (IPC) can be seen as a benefit in reducing the EE magnification, as it can reduce the deleterious effects of ischemia-reperfusion, and can be used to accelerate the post-exercise recovery process. Objectives: the general objective of this study will be to evaluate the behavior of cellular responses (R, Xc and phA) after ICP at different occlusion pressures and correlate them with indirect markers of eccentric muscle damage (CK and LDH). Methods: a randomized placebo-controlled clinical trial will be carried out with 80 healthy men aged 18 to 35 years who will be randomly be divided into four groups: IPC using total occlusion pressure (POT), IPC with 40% more than POT, placebo (10 mmHg) and control. The IPC protocol will consist of four cycles of ischemia and reperfusion of five minutes each. All groups will carry out an EE protocol, initial assessments, immediately after the end of the EE, 24, 48, 72, and 96 hours after exercise, sequentially evaluating creatine kinase (CK), blood lactate (LDH), and complete vector cell through the bioelectrical impedance (BIA). Will be used the descriptive statistical method and analysis of variation for the repeated measures model. The level of significance will be p < 0.05. (AU)