Eccentric training at different intensities associated with blood flow restriction influences autonomic, cellular, functional and clinical responses?: a randomized controlled clinical trial

• Grant number: 20/13143-8
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2021 - March 31, 2023
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Franciele Marques Vanderlei
• Grantee: Franciele Marques Vanderlei
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
• Associated researchers: Allysiê Priscilla de Souza Cavina ; Carlos Marcelo Pastre ; David Matthew Garner ; Eduardo Pizzo Junior ; Leonardo Kesrouani Lemos ; Taíse Mendes Biral
• Associated scholarship(s): 22/06626-8 - Eccentric training at different intensities associated with blood flow restriction influences autonomic, cellular, functional and clinical responses: A randomized controlled clinical trial, BP.TT

Abstract

Studies on low intensity resistance training associated with blood flow restriction (BFR) have gained prominence in recent years, however there are still gaps that can be explored in relation to their physiological effects when associated with eccentric training, in addition to the dose-response relationship hemodynamic and autonomic of BFR associated with different training intensities in post-exercise recovery. Thus, from this exploration, the investigation of eccentric training associated with BFR becomes relevant. Objectives: the primary objective of the study will be to evaluate the behavior of hemodynamic and autonomic parameters and cellular adaptations during sessions and after six weeks of eccentric resistance training of high and low intensity knee extensors associated or not with BFR in young people. And the secondary objectives will be: i) to evaluate the efficacy of the eccentric resistance training of knee extensors of high and low intensity associated or not to the BFR in the strength, hypertrophy and functional performance of young people and, ii) to analyze the clinical, perceptual and recuperative parameters during and after high and low intensity eccentric resistance training associated or not with BFR in young people. Methods: this is a randomized controlled clinical trial including 60 men, aged between 18 and 35 years, randomized into four groups according to the intensity of the exercise and BFR, which are: 80% without BFR, 40% without BFR, 80% with BFR and 40% with BFR. The intensity of the exercise will be determined by the peak eccentric torque assessed by the isokinetic dynamometer and the BFR will be determined as 40% of the pressure necessary for the complete restriction of blood flow being assessed by Doppler. The participant will perform a program of eccentric training of the quadriceps muscle in the isokinetic dynamometer lasting six weeks and the heart rate variability (HRV) outcomes analyzed in the time domain (mean RR, rMSSD and SDNN), frequency domain (LF and HF [nu and ms2]) and Poincaré plot (SD1 and SD2), hemodynamic parameters (heart rate, systolic and diastolic blood pressure, respiratory rate, oxygen saturation and double product), cell integrity (resistance, reactance and phase angle by electrical bioimpedance), muscle strength (eccentric, concentric and isometric peak torque), muscle structure (ultrasound), functional test (Single Leg Hop Test) and subjective perception of pain, effort and recovery will be evaluated one week before, in the fourth week and one week after the end of the training program. In addition, clinical markers of pain, perceived exertion and recovery will be investigated daily before and after the session. Finally, post-exercise recovery analysis will be performed using the recovery heart rate (RHR) in the 1st (RHR1) and 2nd (RHR2) minute, 30-second rMSSD, hemodynamic parameters, HRV índices and segmental and global analysis of electrical bioimpedance. The descriptive statistical method will be used and the comparison between the moments and groups will be carried out using the technique of analysis of variance for the model of repeated measures in the two-factor scheme followed by post hoc tests. The level of significance will be p <0.05. (AU)