Histomorphometric evaluation of the remplanted murine tooth repair process after the application of laser therapy (LLLT)

• Grant number: 21/07291-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2021
• End date: June 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Christine Men Martins
• Grantee: Larissa dos Santos de Moraes
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Trauma injuries to teeth and supporting tissues caused by external forces can lead to intrusion and avulsion. Avulsion promotes the complete exarticulation of the tooth out of its socket. The main therapeutic resource is the immediate reimplantation of the tooth in the alveolus, but when performed late, inflammatory and/or tooth root replacement resorptions may occur. In order to minimize the occurrence of root resorption after reimplantation and to find an ideal surface treatment, this study proposes to evaluate the repair process of dental tissues after late reimplantation using the application of LLLT for the treatment of the surface. Thirty rats were randomly divided into 4 groups and had their upper right central incisors extracted and immersed in different media for 30 min, according to the Negative control group, teeth remained in saline solution; LLLT group, teeth were immersed in saline solution, and, after this period, the teeth were treated with LLLT; and Grupo Milk, teeth immersed in milk. Afterward, the teeth were reimplanted and after 60 days the evaluation with Picrosirius red was performed to analyze the collagen fibers of the periodontal ligament. There was a need to perform histomorphometric analysis by staining with Hematoxylin and Eosin. Therefore, this research is justified. Statistical analysis will be performed with a significance level of 5%. It is believed that the association of laser therapy (LLLT) as a surface treatment obtains better results in the repair process of the periodontal ligament and pulp after late reimplantation when compared to milk. (AU)