Characterization of the H3K9 histone acetylation profile in surgical pathologies of the parathyroid glands

• Grant number: 21/09810-1
• Support Opportunities: Scholarships abroad - Research
• Start date: December 01, 2021
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Luiz Carlos Conti de Freitas
• Grantee: Luiz Carlos Conti de Freitas
• Host Investigator: Rogerio Moraes Castilho
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Michigan, United States

Abstract

Primary hyperparathyroidism is most commonly caused by an adenoma in one of the four parathyroid glands, while about 15 to 20% is caused by hyperplasia, and only 1% is caused by carcinoma. Secondary hyperparathyroidism is largely associated with kidney failure. Histopathological features often do not allow for a precise diagnosis and, in addition, the molecular mechanisms involved in the disease worsening are not fully understood. Epigenetic mechanisms have been studied with the aim of identifying characteristics that differentiate neoplasms and help to elucidate pathophysiological mechanisms, including histone acetylation. The imbalance between histone acetylation and deacetylation seems to favor tumors onset. This study aims to demonstrate the histone 3 (H3K9) acetylation profile in different types of pathologies of the parathyroid glands, such as parathyroid hyperplasia, adenoma and carcinoma. Twenty-five parathyroids samples from patients undergoing parathyroidectomy for hyperparathyroidism between 2010 and 2020 at the Head and Neck Surgery Service of the USP campus in Ribeirão Preto will be analyzed. Clinical, demographic and laboratory data will be collected from the enrolled patients and the profile of histone acetylation in the removed parathyroid tissues as well as in normal parathyroid biopsies will be determined. For this, the samples will be sent to the laboratory of epithelial biology at the University of Michigan, where they will be classified for positivity for H3K9ac, by immunohistochemical detection. Clinical and immunohistochemical data will be evaluated to determine its correlation with the acetylation feature in hyperplasia and adenoma samples. In addition, the application of histone acetylation in the differentiation between benign and malignant neoplasms will be analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONTI DE FREITAS, LUIZ C.; CASTILHO, ROGERIO M.; SQUARIZE, CRISTIANE H.. Histone Modification on Parathyroid Tumors: A Review of Epigenetics. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 10, p. 10-pg., 2022-05-01. (21/09810-1)