Advanced search
Start date

The role of the capsule encoded by the WCB cluster of Burkholderia cenocepacia H111 and B. seminalis TC3.4.2R3 on the microbial interaction

Grant number: 21/09835-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2021
Effective date (End): November 30, 2023
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Welington Luiz de Araújo
Grantee:Elisabeth Fermi Maestá Filippi
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The Burkholderia genus is composed of a broad range of species with phenotypical variations that allow them to occupy different niches, colonizing environments with contrasting characteristics, such as soil, rhizosphere, freshwater, and the inner plant or animal tissues. Burkholderia cepacia complex (Bcc) is a group of species that present a great relevance for medical and agricultural studies since species belonging to this group are associated with biological control of plant disease and inducing inflammatory and pneumonia symptoms in immunocompromised patients. Therefore, the aim of the present study is to understand the molecular and ecological strategy used by these bacteria to interact with plants and animals allowing to development of strategies to control phytopathogens presenting a low risk to animal and human health. The genome of Burkholderia spp. contains a cluster named WCB, which encodes a protein involved in the synthesis of the bacterial capsule and in ecological interactions. Recent studies have shown that this capsule is related to the virulence of Burkholderia spp. and other species, by providing a strategy to evade the host immune system. The ecological interactions of this genus, however, include not only pathogenic ones, but also mutualistic associations with fungi, plants, and animals. The capsule also plays a role in these beneficial interactions, specifically in the colonization of the organisms. Hence, we will generate mutants for genes (glycosyltransferase, transporters, regulators, and lipidic transformation metabolism) belonging to WCB cluster and evaluate the effects of mutations in different mechanisms associated with ecological interactions of Burkholderia cenocepacia H111 and B. seminalis TC3.4.2R3. To generate these mutants, we will use a mutagenesis technique based on the I-Scel nuclease utilization. The present study will provide new insights into the strategies used by these bacteria to colonize different environments and hosts. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list using this form.