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Use of systems biology tools to evaluate the effects of fetal programming in beef cattle

Grant number: 21/03265-1
Support Opportunities:Scholarships in Brazil - Doctorate
Start date: January 01, 2022
End date: December 31, 2026
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Miguel Henrique de Almeida Santana
Grantee:Guilherme Henrique Gebim Polizel
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:17/12105-2 - Fenomics of fetal programming effects on beef cattle production: a systemic approach, AP.JP
Associated scholarship(s):23/09113-4 - Integration of multi-omics functional information to better understand the fetal programming effects on bulls, BE.EP.DR

Abstract

Systems biology is the field that studies the interactions between components of a biological system in a holistic way, however it is still little explored in science due to the difficulty of integrating data from different molecular levels. It is already known that the nutrition of pregnant mothers in livestock is an extrinsic factor that can interfere with characteristics of productive interest throughout the life of the offspring, however the mechanisms related to the effects of this highly complex process still require studies. This project seeks to elucidate part of the mechanisms for multiomic and tissue integration in male Nellore animals that have been identified with different prenatal supplementation strategies. This study will be based on 63 animals from 3 different nutritional prenatal treatments: NP - only mineral supplementation (common to all treatments), PP - protein-energy supplementation in the final third of pregnancy and PC - protein-energy supplementation throughout the gestation. This project is divided into 3 studies. In study 1, a multi-omic approach of liver from selected animals will be carried out. The differential expression and co-expression networks of the genes will be evaluated by transcriptomics. The metabolites present in that organ will be evaluated by the metabolomics. These data will integrate with genomic data of 35 thousand single nucleotide polymorphisms (SNPs) to relate to the performance of these bulls. In study 2, the systems biology approach using differential expression and gene co-expression networks in the liver and skeletal muscle of bulls will be carried out by transcriptomics, the profile of metabolites in plasma, meat, subcutaneous fat and liver will be evaluated by metabolomics and profile of ruminal microbial communities will be evaluated by metagenomics. In this study, fetal programming will be associated with multi-tissue transcriptomics and multi-tissue metabolomics and the integration of omics, tissues and phenotypes of bulls from birth to slaughter. This project may demonstrate the biological basis of the influence of fetal programming on the performance of Nellore animals. (AU)

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Scientific publications (6)
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
POLIZEL, GUILHERME HENRIQUE GEBIM; FERNANDES, ARICIA CHRISTOFARO; FURLAN, EDISON; PRATI, BARBARA CAROLINA TEIXEIRA; FERRAZ, JOSE BENTO STERMAN; SANTANA, MIGUEL HENRIQUE DE ALMEIDA. Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase. METABOLITES, v. 13, n. 2, p. 14-pg., . (21/03265-1, 17/12105-2)
GEBIM POLIZEL, GUILHERME HENRIQUE; CORREIA QUEIROZ CANCADO, FERNANDO AUGUSTO; FERREIRA DIAS, EVANDRO FERNANDO; FERNANDES, ARICIA CHRISTOFARO; CRACCO, ROBERTA CAVALCANTE; CARMONA, BRENDA TONSIC; CASTELLAR, HENRIQUE HESPANHOL; POLETI, MIRELE DAIANA; DE ALMEIDA SANTANA, MIGUEL HENRIQUE. Effects of Different Prenatal Nutrition Strategies on the Liver Metabolome of Bulls and Its Correlation with Body and Liver Weight. METABOLITES, v. 12, n. 5, p. 13-pg., . (17/12105-2, 21/03265-1)
POLIZEL, GUILHERME HENRIQUE GEBIM; FANALLI, SIMARA LARISSA; DINIZ, WELLISON J. S.; CESAR, ALINE SILVA MELLO; CONSOLO, NARA REGINA BRANDAO; FUKUMASU, HEIDGE; CANOVAS, ANGELA; FERNANDES, ARICIA CHRISTOFARO; PRATI, BARBARA CAROLINA TEIXEIRA; FURLAN, EDISON; et al. Liver transcriptomics-metabolomics integration reveals biological pathways associated with fetal programming in beef cattle. SCIENTIFIC REPORTS, v. 14, n. 1, p. 20-pg., . (21/03265-1, 17/12105-2)
POLIZEL, GUILHERME HENRIQUE GEBIM; DINIZ, WELLISON J. S.; CESAR, ALINE SILVA MELLO; RAMIREZ-ZAMUDIO, GERMAN D.; CANOVAS, ANGELA; DIAS, EVANDRO FERNANDO FERREIRA; FERNANDES, ARICIA CHRISTOFARO; PRATI, BARBARA CAROLINA TEIXEIRA; FURLAN, EDISON; POMBO, GABRIELA DO VALE; et al. Impacts of prenatal nutrition on metabolic pathways in beef cattle: an integrative approach using metabolomics and metagenomics. BMC Genomics, v. 26, n. 1, p. 21-pg., . (21/03265-1, 17/12105-2)
POLIZEL, GUILHERME HENRIQUE GEBIM; SANTOS, MARIA ELIS PERISSIN DOS; CESAR, ALINE SILVA MELLO; DINIZ, WELLISON J. S.; RAMIREZ-ZAMUDIO, GERMAN D.; FANTINATO-NETO, PAULO; FERNANDES, ARICIA CHRISTOFARO; PRATI, BARBARA CAROLINA TEIXEIRA; FURLAN, EDISON; POMBO, GABRIELA DO VALE; et al. Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle. VETERINARY SCIENCES, v. 12, n. 5, p. 14-pg., . (17/12105-2, 21/03265-1)
GEBIM POLIZEL, GUILHERME HENRIQUE; ESPIGOLAN, RAFAEL; FANTINATO-NETO, PAULO; STREFEZZI, RICARDO DE FRANCISCO; RANGEL, RAISSA BRAIDO; DE CARLI, CYNTHIA; FERNANDES, ARICIA CHRISTOFARO; FERREIRA DIAS, EVANDRO FERNANDO; CRACCO, ROBERTA CAVALCANTE; DE ALMEIDA SANTANA, MIGUEL HENRIQUE. Different prenatal supplementation strategies and its impacts on reproductive and nutrigenetics assessments of bulls in finishing phase. VETERINARY RESEARCH COMMUNICATIONS, v. N/A, p. 15-pg., . (17/12105-2, 21/03265-1)