Design and production of bone vascular scaffolds

• Grant number: 21/11803-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: October 01, 2022
• End date: September 30, 2023
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Antonio Claudio Tedesco
• Grantee: Henrique Luis Piva
• Supervisor: Ibrahim Tarik Ozbolat
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Pennsylvania State University, United States
• Associated to the scholarship: 20/04009-6 - Development of a novel bioink for 3D printing scaffolds for regeneration of cartilage tissues, BP.DD

Abstract

Research on novel compatible biomaterials for three-dimensional (3D) bioprinting exhibiting fast crosslinking properties and multiple advanced functions (like good cell viability, transparency, exhibiting good cell fusion potential) used as scaffolds is essential for advances in customized applications in many research fields, including tissue engineering. Many studies have explored such biomaterials to improve its functionality, however, there is a lack of vascularization process in scaffolds for implants, this is an essential factor for successful implants of bioengineered tissues, and a tissue with few millimeters in volume cannot survive by diffusion for oxygen and nutrients, requiring then a formation of capillaries to supply it. This proposal of study will use the combination of Gellan-Gum, and Alginate as bioinks in a gelatin-based hydrogel to produce vascularized scaffolds with human umbilical vein endothelial cells (HUVECs), combined with a PVP and gelatin-based hydrogel containing human fetal osteoblasts for future bone regeneration processes, avoiding the lack of vascularization. (AU)