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Response of bovine luminal endometrial epithelial cells to stimulus provided by bovine trophoblast cells and conjugated linoleic acid in a co-culture system

Grant number: 21/14530-8
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): July 01, 2022
Effective date (End): June 30, 2023
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Marcelo Fábio Gouveia Nogueira
Grantee:Mariângela Bueno Cordeiro Maldonado
Supervisor: Mario Binelli
Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
Research place: University of Florida, Gainesville (UF), United States  
Associated to the scholarship:19/00637-5 - Effect of supplementation with conjugated linoleic acid in culture of trophoblastic cells and in vitro production of bovine embryos on synthesis of prostaglandin E2 and F2α and expression of transcripts involved in eicosanoid biosynthesis and establishment, BP.PD


Early embryonic mortality, caused by failures in maternal-fetal recognition in the first three weeks after fertilization represents a major cause of reproductive inefficiency in the cattle industry. During this pre-implantation period, the conceptus (embryo and its associated extra-embryonic membranes) present in the uterine lumen must secrete molecules, such as interferon-tau (IFNT), that interact with the endometrium to inhibit prostaglandin F2± synthesis and luteolysis, a determining condition for the establishment of pregnancy. Mechanisms regulated by conceptus IFNT in bovine uterine epithelial cells (BUECs) include the upregulation of transcription of IFN-stimulated genes (ISGs). A major gap in the current knowledge on the field of pre-implantation biology in cattle is the nature of one-to-one endometrium and embryo interactions that result in positive or negative pregnancy outcomes. Understanding such interactions will serve as basis to develop strategies that can benefit the maternal-embryo recognition, which are of scientific and economic interest. However, models to study the embryo-endometrium interactions in vitro are limited. The main objective is to develop and validate an in vitro co-culture model to determine the response of BUECs to a bovine trophoblast cell line (CT-1) and to exogenous stimuli, such as conjugated linoleic acid (CLA). Here, we propose to characterize the transcription of ISGs, such as ISG15, OAS1, and MX2 in BUECs, after co-culture with CT-1 and exposure to CLA. The hypothesis is that BUECs derived from different cows respond differently to the stimuli provided by CT-1 and CLA. Contrasting responses among different cows is expected to be associated with different individual-cow endometrial receptivity to implantation as well as survival, growth, and development of the conceptus. (AU)

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