Evaluation of adrenergic beta antagonists in the vas deferens duct in rats

• Grant number: 21/14120-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2022
• End date: December 31, 2022
• Field of knowledge: Biological Sciences - Pharmacology - Autonomic Pharmacology
• Principal Investigator: Gilberto de Nucci
• Grantee: Raquel Rios Campitelli
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 19/16805-4 - Evaluation of the pathophysiological role of endothelial catecholamines, AP.TEM

Abstract

Electric field stimulation (EFS) is a technique in which an electrical stimulus is applied uniformly to isolated tissue in short-wave pulses. Depending on the mediators released by the EFS, tissue contraction or relaxation may occur. Initially, our research group demonstrated that stimulated contractions of human umbilical cord vessels (HUCV) depend on the presence of the endothelium and are not affected by tetrodotoxin, a sodium channel blocker used to prevent the release of mediators from nerve endings. Similar findings were found in reptiles. aorta of the tortoise Chelonoidis carbonaria and aorta of Crotalus durissus terrificus, Bothrops jararaca and Panterophis guttatus. Recently, the basal release of dopamine by HUCV endothelial cells was detected through pharmacological assays and by liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS). Dopamine acts in dopaminergic neurotransmission in the central nervous system, in the modulation of blood pressure by renal and vascular mechanisms, and is important in the modulation of cardiac function. LC-MS-MS analyzes also revealed a new vascular mediator to 6-nitrodopamine. 6-nitrodopamine is produced from the nitration of dopamine. In studies, our research group identified the release of 6-nitrodopamine in the vas deferens duct in rats. This work aims to characterize the action of 6-nitrodopamine in beta adrenergic receptors in the vas deferens duct in rats. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, ANTONIO TIAGO; AMORIM, AMANDA CONSULIN; BRITTO-JUNIOR, JOSE; CAMPITELLI, RAQUEL RIOS; FREGONESI, ADRIANO; MONICA, FABIOLA Z.; ANTUNES, EDSON; DE NUCCI, GILBERTO. beta(1)- and beta(1)/beta(2)-adrenergic receptor antagonists block 6-nitrodopamine-induced contractions of the rat isolated epididymal vas deferens. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, v. 395, n. 10, p. 12-pg., 2022-07-08. (21/14120-4, 19/16805-4, 17/15175-1, 21/13593-6)