The impact of testosterone on the orthodontic tooth movement: an in vivo study

• Grant number: 21/02704-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): April 01, 2022
• Effective date (End): December 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Mírian Aiko Nakane Matsumoto
• Grantee: Caio Luiz Bitencourt Reis
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Pubertal growth spurt can modify the patient's response to Orthodontic Tooth Movement (OTM). However, the role of testosterone in OTM was little studied. This study aims to investigate the impact of suppression, replacement, and supraphysiological doses of testosterone during OTM in animal models. 23-day-old Wistar male rats (n=72) will be randomly allocated into four groups. Orchiectomy (ORX) will be performed in two groups, and, in one group, Testosterone Undecanoate (TU) will be administered for replacement. Placebo Surgery (Sham) will be made in two groups, and, in one group, will be administered supraphysiological doses of TU. At 50-day-old, a NiTi closed coil spring will induce the OTM of the first left upper molar in a constant orthodontic force of 0.25 N. The right side of the maxilla will be the control side. The animals will be euthanized after 5 and 10 days of OTM. Images of histological slices will be obtained and stained with HE to inflammation analysis, Red Sirius to collagen analysis and TRAP to osteoclastogenisis analysis. Immunohistochemical and gene expression by RT-qPCR of RANKL, OPG, RUNX2, BMP-2 e VEGF will be performed. The oxidative stress will be assessed by endothelial NOS immunohistochemical, and apoptosis by TUNEL method. Cardiac function will be evaluated by Troponin I serum and adrenal gland by Adrenocorticotropic hormone. Bone quality and volume of root resorption will be evaluated by 3D microtomography. Data will be analyzed at a significance level of 0.05. This study has the potential to generate unpublished results and will be directly applicable in the orthodontic treatment of teenagers because it aims to assess the need to investigate hormonal levels before starting the orthodontic treatment. (AU)

Publicações científicas

(4)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

REIS, CAIO LUIZ BITENCOURT; PINGUEIRO-OKADA, ELAINE MACHADO; LUIZ, KELLY GALISTEU; PEDROSO, GABRIELA LEITE; MATSUMOTO, MIRIAN AIKO NAKAME; DE MENEZES, LUCIANE MACEDO; KUECHLER, ERIKA CALVANO; NASCIMENTO, GLAUCE CRIVELARO; STUANI, MARIA BERNADETE SASSO. Orthodontic pain: c-Fos expression in rat brain nuclei after rapid maxillary expansion. JOURNAL OF THE WORLD FEDERATION OF ORTHODONTISTS, v. 12, n. 1, p. 6-pg., 2023-02-01. (21/02704-1)

ISABELA RIBEIRO MADALENA; CAIO LUIZ BITENCOURT REIS; MIRIAN AIKO NAKANE MATSUMOTO; MARIA BERNADETE SASSO STUANI; NATANAEL HENRIQUE RIBEIRO MATTOS; DANIELA SILVA BARROSO DE OLIVEIRA; MARIA ANGÉLICA HUEB DE MENEZES OLIVEIRA; LILIANE ROSKAMP; ERIKA CALVANO KÜCHLER; FLARES BARATTO-FILHO. Investigating the association between dental age and polymorphisms in genes encoding estrogen receptors. Journal of Applied Oral Science, v. 31, 2023-09-25. (21/02704-1)

REIS, CAIO LUIZ BITENCOURT; MARANON-VASQUEZ, GUIDO ARTEMIO; MATSUMOTO, MIRIAN AIKO NAKANE; BARATTO-FILHO, FLARES; STUANI, MARIA BERNADETE SASSO; PROFF, PETER; KIRSCHNECK, CHRISTIAN; KUECHLER, ERIKA CALVANO. Single nucleotide polymorphisms in odontogenesis-related genes associated with tooth-size discrepancy. AUSTRALASIAN ORTHODONTIC JOURNAL, v. 39, n. 1, p. 10-pg., 2023-01-01. (21/02704-1)

BITENCOURT REIS, CAIO LUIZ; MATSUMOTO, MIRIAN AIKO NAKANE; BARATTO-FILHO, FLARES; SCARIOT, RAFAELA; STUANI, MARIA BERNADETE SASSO; LOURENCO ROMANO, FABIO; DELLA COLETTA, RICARDO; DE OLIVEIRA, DANIELA SILVA BARROSO; PROFF, PETER; KIRSCHNECK, CHRISTIAN; et al. Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study. Head & Face Medicine, v. 19, n. 1, p. 8-pg., 2023-07-03. (21/02704-1)