Generation of microfluidic devices with different geometries for in vitro fertilization

• Grant number: 22/02701-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2022
• Status: Discontinued
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Juliano Coelho da Silveira
• Grantee: Franciele Flores Vit
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 21/06645-0 - Extracellular vesicles as a platform for diagnostic and manipulation of the in vitro embryo production system: the next generation in animal reproduction, AP.JP2
• Associated scholarship(s): 25/00727-5 - Development of microfluidic devices for the isolation and analysis of extracellular vesicles from spent embryo culture media, BE.EP.PD

Abstract

This proposal has as main objective the development of microfluidic devices to evaluate the quality of bovine embryos. The aim is to dynamically observe the embryonic process and the quality of the embryos. To assess the quality of the embryos, the culture medium will be collected for 3 days and the extracellular vesicles (EVs) present in this medium will be characterized. The quality of embryos in microdevices and in the conventional method (macroscale) will be estimated by analyzing sizes, loads, concentration of miRNA and DNA present in the EVs. For this, the microdevices will first be operationally and biologically validated (biocompatibility), concomitantly extracting the oocytes and characterizing them. After that, the oocytes will be fertilized (embryo formation) in microdevices and in plates for 3 days. During this period, samples will be taken from the culture medium and the EVs resulting from the embryonic process will be characterized. Through the characteristics of these EVs, it will be possible to determine good quality embryos. The results obtained in microdevices will be statistically analyzed and compared to the conventional system (macroscale).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRONATO, GIULIANA DE AVILA; VIT, FRANCIELE FLORES; DA SILVEIRA, JULIANO COELHO. 3D culture applied to reproduction in females: possibilities and perspectives. ANIMAL REPRODUCTION, v. 21, n. 1, p. 18-pg., 2024-01-01. (21/06645-0, 22/02701-5, 19/25675-7)

GIULIANA DE AVILA FERRONATO; FRANCIELE FLORES VIT; JULIANO COELHO DA SILVEIRA. 3D culture applied to reproduction in females: possibilities and perspectives. Animal Reproduction, v. 21, n. 1, 2024-03-08. (21/06645-0, 22/02701-5, 19/25675-7)