Transcriptional profiles of embryo competence

• Grant number: 22/02139-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2022
• End date: March 31, 2026
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Mateus José Sudano
• Grantee: Andressa Minozzo Oliveira
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 18/21496-8 - Pathways for embryo-derived pregnancy, AP.JP

Abstract

The pathways and biological processes associated with embryo competence, cryosurvival and the success of obtaining an embryo-derived pregnancy are still lacking. The present work aims to elucidate strategies and understand the mechanisms of the competence of In Vitro Produced (IVP) bovine embryos in a holistic approach. In a series of three experiments, we will look for: 1) identify differentially expressed molecules between Inner Cell Mass (ICM) and trophectoderm (TE) cells from embryos that resulted in positive and negative pregnancies for the establishment of the transcriptional profiles and pathways that favor embryo-derived pregnancy success according to the cell type; 2) understand the functional role of facilitating pregnancy molecules according to cell type (ICM and TE) during early embryo development; 3) establish an embryo culture medium that favors the activation of facilitating pregnancy molecules and pathways in order to improve embryo in vitro production system. Multivariate and univariate statistical models will be used in robust softwares capable of analyse a great amount of data and provide solid answer to the tested hypothesis. The significance level of 5% (P<0.05) will be used. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARSICO, THAMIRIS VIEIRA; VALENTE, RONIELE SANTANA; ANNES, KELLY; OLIVEIRA, ANDRESSA MINOZZO; SILVA, MARA VIANA; SUDANO, MATEUS JOSE. Species-specific molecular differentiation of embryonic inner cell mass and trophectoderm: A systematic review. Animal Reproduction Science, v. 252, p. 11-pg., 2023-04-18. (19/17796-9, 18/21496-8, 20/08826-9, 22/02139-5, 19/18415-9)

MINOZZO, OLIVEIRA ANDRESSA; VIEIRA, MARSICO THAMIRIS; JOSE, SUDANO MATEUS. Transverse and vertical incisions affect the viability of in vitro-produced embryos submitted to a simplified microsurgery approach. Theriogenology, v. 226, p. 8-pg., 2024-07-02. (21/01446-9, 22/02139-5, 18/21496-8, 19/17796-9)