In vitro maternal-embryary interaction mediated by extracellular vesicles in cattle

• Grant number: 21/02458-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2022
• Effective date (End): February 28, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Flávio Vieira Meirelles
• Grantee: Amanda Nespolo Silva
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In cattle, pregnancy failures occur more frequently during the period embryonic compared to the fetal or neonatal period. About,40% of all embryonic losses are associated with failures without maternal recognition prior to the 21st day of pregnancy. The acknowledgment maternal pregnancy is the period when the concept signals its presence for the mother. The production and release of EVs is an important mechanism for cellular communication present in several types of cells. However, its importance in the reproductive system and also in mediating interactions maternal-embryonic during pregnancy was only recognized recognized. This Therefore, the objective of this project is to generate an in vitro model of communication maternal-embryonic to study the mechanisms of EV release by endometrial cells (epithelial and stromal) and by the embryo (cells of the trofectoderme, TE), as well as the different components of information contained in the EVs. Therefore, the central hypothesis of this project is that endometrial cells and TE from bovine embryos sought for IVF are capable of synthesizing and releasing extracellular vesicles that act in the maternal-embryonic communication. To test this hypothesis we will develop an in vitro culture of endometrial and embryonic cells for the study of the communication processes between these different types of cell phones.After this process, the EVs present in the cultivation of endometrial cells and ET cells. Later, we will travel endometrial cells with EVs produced by TE cells and also do the reverse treatment, where the embryonic cells will be treated with as maternal EVs. The purpose of this study is to better understand the importance that EVs can have in the regulation of biological processes needed during the establishment of pregnancy, such as: angiogenesis, remodeling of the matrix extracellular, changes in immune function and pathological effects in diseases gestational. In addition, the development of this project will assist us in better understanding of maternal-embryonic communication, enabling thus, the improvement of assisted reproductive technologies. (AU)