Epigenetic variation related to the degree of helmintosis in Nelore cattle

• Grant number: 22/00061-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2022
• End date: February 28, 2023
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Ricardo Velludo Gomes de Soutello
• Grantee: Isabela de Almeida Cipriano
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil

Abstract

Given the importance of controlling gastrointestinal nematodes in cattle, it is necessary to search for alternatives such as the use of resistant animals and the diversity of the host genome. Therefore, research on epigenetic mechanisms can be used as a tool for the identification and possible selection of resistant animals. Thus, the objective is to analyze the global DNA methylation content and the existence of epigenetic variability, correlating with the degree of helminth in 60 contemporary Nellore steers, kept on pasture, evaluated monthly for one year by means of egg counting per gram of feces (OPG). The mean OPG counts will be submitted to analysis of variance (ANOVA), considering three classifications (resistant, resilient and susceptible) and subsequent statistical analysis by the Scott-Knott test (5%) to assess the percentage of animals within each classification by through the SISVAR programs. From the DNA extracted from the blood of the steers, the global genomic methylation of the animals will be analyzed, as well as the epigenomic profile of each one using the MSAP technique. The epigenomic data, along with the biometric data, will be subjected to principal component analysis using the residual correlation matrix obtained by the joint analysis of variance using the GENES program. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CIPRIANO, Isabela de Almeida. Epigenetic variation related to the degree of helminthosis in nelore cattle. 2023. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Tecnológicas. Dracena Dracena.