Immunohistochemical study of the inflammatory reaction produced by bacterial cellulose experimentally implanted in the peritoneum of Wistar rats

• Grant number: 22/02480-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2022
• End date: April 30, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Alessandre Hataka
• Grantee: Anna Júlia de Souza Porto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Traumatic injuries involving tissue frayed and loss in domestic animals are frequent, and in several situations, tissue healing recovery or reconstruction are not possible due to their extent, and the use of bioimplants is necessary. Bacterial cellulose (BC) has been used for many applications in human medicine. However, in veterinary medicine, studies investigating the morphological and immunohistochemical characteristics of the inflammatory and scar component in CB implanted in the peritoneum in vivo have not been observed. And even today there is controversy about CB's biocompatible to the inflammatory and scarring response. The aim of this study is to evaluate the inflammatory reaction generated by intraperitoneally implanted CB membrane in Wistar rats, by means of histopathology and immunohistochemistry of lymphocyte types at the implantation site. For this purpose, 30 male or female Wistar rats will be surgically implanted with CB membranes in the peritoneal cavity, 15 animals will receive the CB and 15 will be the Sham group. Sixty days postoperatively, the implant sites will be harvested for histopathological and immunohistochemical analysis. The inflammatory and cicatricial response around the CB will be evaluated, as well as the intensity and type of inflammatory infiltrate by histopathology and by immunohistochemistry. Then the types of lymphocytes T (CD3) or B (CD20) in the process will be characterized. The results obtained will be plotted and statistically analyzed to verify whether there are significant differences.