Antiviral, antimicrobial, micromechanical properties and surface characteristics of the polymethylmethacrylate for prosthesis bases obtained by 3D printing and incorporated with nanostructured silver vanadate decorated with silver nanoparticles

• Grant number: 20/10575-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2022
• End date: August 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Andréa Cândido dos Reis
• Grantee: Ana Beatriz Vilela Teixeira
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The antiviral and antimicrobial capacity of dental materials can be achieved by incorporating nanoparticles. The nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) when incorporated to acrylic resins showed promising results. In addition to the conventional technique, the manufacture of prostheses by 3D printing streamlines the process and obtains devices with greater precision. The modification of PMMA for 3D printing with AgVO3 is an unprecedented proposal that, in addition to reducing the formation of biofilm, can present an antiviral potential against SARS-CoV-2, preventing local and systemic diseases. Associated with the effectiveness of the nanomaterial, the evaluation of the surface characteristics and micromechanical properties can contribute to the selection of the best method for obtaining the prosthesis. The objective of this study is to incorporate AgVO3 into PMMA for 3D printing and heat-curing, in concentrations of 1%, 2.5%, and 5%, and to evaluate the antiviral, antimicrobial activity, surface characteristics, and micromechanical properties. The antiviral capacity will be evaluated by high-throughput immunoplate assay (iPA) optimized for SARS-CoV-2. The antimicrobial activity will be evaluated against two types of multispecies biofilm. The surface characteristics will be verified by wettability in a goniometer, and the morphology, roughness and strength curve of the surface in an Atomic Force Microscope (AFM). Micromechanical properties will be evaluated by nanoindentation in AFM. After the analysis, the distribution of the data will be verified, and then parametric, non-parametric tests or descriptive analysis will be applied with a significance level of 5%. (AU)