The influence of high-intensity interval training on vasomotor and angiogenic molecular markers of skeletal muscle of hypertensive: experimental study and bioinformatics analysis

• Grant number: 22/05715-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2022
• Effective date (End): August 31, 2023
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Francis Lopes Pacagnelli
• Grantee: Helen Louisi Camilo
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Hypertension is a serious risk factor for cardiovascular and renal diseases and causes muscular vascular alterations that compromise exercise capacity and worsen quality of life. HIIT (High Intensity Interval Training) is a safe, low cost, and easy to perform non-pharmacological therapy indicated for hypertensive patients. However, there are no studies evaluating this type of exercise on gene expression of endothelial markers in skeletal muscle. Objective: To evaluate the influence of HIIT on molecular markers of vasomotor and angiogenic activity in the soleus and diaphragm muscles of spontaneously hypertensive rats and to compare, by bioinformatics analysis, the results of studies in humans using the same approach. Methods: We used (n=30) 3-month-old male rats divided into three groups: normotensive Wistar Kyoto sedentary (WKY, n10) spontaneously hypertensive sedentary control (SHR-SED, n=9) and spontaneously hypertensive trained rats (SHR+HIIT, n=11). Blood pressure was checked before and after the training period. The training protocol was performed on a treadmill adapted for rodents (model TK 1, IMBRAMED), 50 minutes/day, 5 days/week totalizing 8 weeks. The rats were submitted to a test to evaluate the maximum speed of exhaustion. Gene expression of VEGF (vascular endothelial growth factor), PI3KR2 (regulatory subunit 2 of phosphatidylinositol 3,4,5 triphosphate), nitric oxide synthase (NOS3) and endothelin (ET-1) genes will be determined by Real-Time PCR technique in soleus and EDL muscles. Thus, in order to complement the data analysis, bioinformatics analysis will be used, with the search of transcriptomic data in the National Center for Biotechnology Information (NCBI) of hypertensive people submitted to HIIT that address angiogenic and vasomotor aspects, for comparison and integration as our results. The aim of this research is to understand the molecular mechanisms involved in HIIT.(AU)