Rankl on the expression of mitochondrial biogenesis markers in miotubes

• Grant number: 22/06359-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2022
• Effective date (End): February 28, 2023
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Mariana Kiomy Osako
• Grantee: Maria Eduarda Ramos Cezine
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The bone-muscle operational unit refers to the mechanical interaction between bone and muscle and the communication via soluble factors that reciprocally regulate their metabolism. RANKL (receptor activator of NFkB ligand) and OPG (osteoprotegerin) are examples of bone-specific proteins that, together with RANK (receptor activator of NFkB), are essential for osteoclast activation and bone resorption in processes of bone remodeling. Especially those involving mitochondrial biogenesis and an increase in oxidative capacity. Our group has recently shown the function of the RANK-RANKL signaling to increase the mitochondrial volume and differentiation of beige adipocytes, a process described as browning. Preliminary data from our lab indicates the role of RANK-RANKL in metabolism control and energy efficiency and its contribution to functions related to the mitochondrial dynamics in the skeletal muscle cells after the RANKL treatment. Mitochondrial dynamics events, such as fusion, fission, and biogenesis, are regulated by PGC1-z expression, which is a transcription factor essential for increasing mitochondrial content and adaptation to energetic demand in the skeletal muscle tissue. Therefore, this project aims to analyze the effect of RANKL on the gene expression of mitochondrial biogenesis markers in myotubes derived from the cell line C2C12 and its impact on metabolism control and energy efficiency.(AU)